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Isolation and characterization of microsatellite markers for carolina hemlock (Tsuga caroliniana)Author(s): S.A. Josserand; K.M. Potter; C.S. Echt; C.D. Nelson
Source: Molecular Ecology Resources, Vol. 8: 1371-1374
Publication Series: Miscellaneous Publication
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DescriptionWe describe the isolation and characterization of 31 polymorphic di- and trinucleotide microsatellite marker loci for Carolina hemlock (Tsuga caroliniana Englem.). In addition, primer pairs for 16 loci amplified scoreable alleles in six other Euga species. In eastern North America, both Carolina hemlock and eastern hemlock (Tsuga canadensis 1L.I Carr.) populations are declining due to infestation by hemlock woolly adelgid, Adelges tsugae. The markers described here should enhance population genetic studies of hemlocks, providing valuable information for conserving and restoring these important forest tree species.
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CitationJosserand, S.A.; Potter, K.M.; Echt, C.S.; Nelson, C.D. 2008. Isolation and characterization of microsatellite markers for carolina hemlock (Tsuga caroliniana). Molecular Ecology Resources, Vol. 8: 1371-1374
Keywordsconservation genetics, hemlock, SSR markers
- Impacts of hemlock decline and ecological considerations for hemlock stand restoration following hemlock woolly adelgid outbreaks
- Evaluating Southern Appalachian forest dynamics without eastern hemlock: consequences of herbivory by the hemlock wooly adelgid.
- Evaluation of hemlock (Tsuga) species and hybrids for resistance to Adelges tsugae (Hemiptera: Adelgidae) using artificial infestation
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