Skip to Main Content
Sulfite pretreatment (SPORL) for robust enzymatic saccharification of spruce and red pineAuthor(s): J.Y. Zhu; X.J. Pan; G.S. Wang; R. Gleisner
Source: Bioresource technology. Vol. 100 (2009): Pages 2411-2418.
Publication Series: Miscellaneous Publication
PDF: Download Publication (299 KB)
DescriptionThis study established a novel process using sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) for robust and efficient bioconversion of softwoods. The process consists of sulfite treatment of wood chips under acidic conditions followed by mechanical size reduction using disk refining. The results indicated that after the SPORL pretreatment of spruce chips with 8-10% bisulfite and 1.8-3.7% sulfuric acid on oven dry (od) wood at 180 °C for 30 min, more than 90% cellulose conversion of substrate was achieved with enzyme loading of about 14.6 FPU cellulose plus 22.5 CBU beta-glucosidase per gram of od substrate after 48 h hydrolysis. Glucose yield from enzymatic hydrolysis of the substrate per 100 g of untreated od spruce wood (glucan content 43%) was about 37 g (excluding the dissolved glucose during pretreatment). Hemicellulose removal was found to be as critical as lignin sulfonation for cellulose conversion in the SPORL process. Pretreatment altered the wood chips, which reduced electric energy consumption for size reduction to about 19 Wh/kg od untreated wood, or about 19 g glucose/Wh electricity. Furthermore, the SPORL produced low amounts of fermentation inhibitors, hydroxy-methylfurfural (HMF) and furfural, of about 5 and 1 mg/g of untreated od wood, respectively. In addition, similar results were achieved when the SPORL was applied to red pine. By building on the mature sulfite pulping and disk refining technologies already practiced in the pulp and paper industry, the SPORL has very few technological barriers and risks for commercialization.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationZhu, J. Y.; Pan, X. J.; Wang, G.S.; Gleisner, R. 2009. Sulfite pretreatment (SPORL) for robust enzymatic saccharification of spruce and red pine. Bioresource Technology. 100: 2411-2418.
KeywordsEnzymatic hydrolysis, size reduction, softwood, sulfite pretreatment, SPORL, pretreatment, enzymes, biotechnology, industrial applications, lignocellulose, biodegradation, spruce, cellulose, hydrolysis, glucose, hemicellulose, fermentation, alcohol, fuelwood, biomass energy, sulfuric acid, energy consumption, furfural, red pine, wood chips, biomass utilization, sugars
- Sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) for robust enzymatic saccharification of hardwoods
- On energy consumption for size-reduction and yields from subsequent enzymatic saccharification of pretreated lodgepole pine
- Comparisons of SPORL and dilute acid pretreatments for sugar and ethanol productions from aspen
XML: View XML