Skip to Main Content
Random amplified polymorphic DNA markers tightly linked to a gene for resistance to white pine blister rust in sugar pineAuthor(s): Michael E. Devey; Annette Delfino-Mix1; Bohun B. Kinloch; David B. NEALEt
Source: Prac. Nail. Acad. Sci. USA Vol. 92, p. 2066-2070
Publication Series: Miscellaneous Publication
PDF: Download Publication (1.0 MB)
DescriptionWe have genetically mapped a gene for resistance to white pine blister rust (Cronartium ribicola Fisch.) in sugar pine (Pinus lambertiana Dougl.) by using an approach which relies on three factors: (i) the ability to assay for genetic markers in the haploid stage of the host's life cycle, using megagametophyte seed tissue; (ii) a simple and clearly defined pathosystem; and (iii) the use of random amplified polymorphic DNA (RAPD) markers that can be quickly and efficiently evaluated. Resistance to white pine blister rust in sugar pine is known to be controlled by a single dominant gene (R). Maternal segregation of R and dominant RAPD markers were scored simultaneously following collection of megagametophytes for DNA assays and seedling inoculation with C. ribicola. Bulked samples of haploid megagametophyte DNA from resistant and susceptible offspring of segregating full-sib and half-sib families were used to evaluate 800 random decanucleotide primers. Ten loci linked with the gene for resistance to white pine blister rust were identified and segregation data were obtained from five families. Six of the linked markers were within 5 centimorgans of the gene, and one marker was 0.9 centimorgan from R. These and other markers derived by this approach may provide starting points for map-based cloning of this important gene.
- You may send email to firstname.lastname@example.org to request a hard copy of this publication.
- (Please specify exactly which publication you are requesting and your mailing address.)
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationMichael E. Devey*, Annette Delfino-Mix1, Bohun B. Kinloch, jR.t, and David B. NEALEt 1995. Random amplified polymorphic DNA markers tightly linked to a gene for resistance to white pine blister rust in sugar pine. Prac. Nail. Acad. Sci. USA Vol. 92, p. 2066-2070
- A sugar pine consensus map: Comparative mapping between the Pinus subgenus Pinus and the subgenus Strobus
- Current and future molecular approaches to investigate the white pine blister rust pathosystem
- White pine blister rust hazard rating for 265 sites in southern Oregon, USA
XML: View XML