Analysis of cellulase and polyphenol oxidase production by southern pine beetle associated fungiAuthor(s): Abduvali Valiev; Zumrut B. Ogel; Dier D. Klepzig
Source: Symbiosis, Vol. 49: 37-42
Publication Series: Miscellaneous Publication
PDF: View PDF (1.07 MB)
In this study, the production of extracellular enzymes by fungi associated with southern pine beetle was investigated for the first time. Cellulase and polyphenol oxidase production were analyzed for three beetle associated fungi. Only the mutualistic symbiont Entomocorticium sp. A was found to produce cellulases and polyphenol oxidase. In time course analyses of cellulase production in batch cultures, Entomocorticium sp. A showed maximum activity of 0.109 U/ml and 0.141 U/ml for total cellulase and endoglucanase activity respectively. Polyphenol oxidase production was simultaneous with fungal growth. Characterization of polyphenol oxidase by activity staining suggests that the enzyme is a tyrosinase/catechol oxidase. Enzyme assays in the presence of polyphenol oxidase inhibitors support the results of the activity staining.
- You may send email to email@example.com to request a hard copy of this publication.
- (Please specify exactly which publication you are requesting and your mailing address.)
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationValiev, Abduvali; Ogel, Zumrut B.; Klepzig, Dier D. 2009. Analysis of cellulase and polyphenol oxidase production by southern pine beetle associated fungi. Symbiosis, Vol. 49: 37-42
KeywordsCeratocystiopsis, Entomocorticium, Ophiostoma, cellulase, tyrosinase/catechol oxidase
- From Attack to Emergence: Interactions between Southern Pine Beetle, Mites, Microbes, and Trees
- Effects of tree phytochemistery on the interactions among endophloedic fungi associated with the southern pine beetle
- Symbiosis and competition: complex interactions among beetles, fungi, and mites
XML: View XML