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Isolation and characterization of 16 microsatellite loci in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae: Scolytinae)Author(s): C. S. Davis; K. E. Mock; B. J. Bentz; S. M. Bromilow; N. V. Bartell; B. W. Murray; A. D. Roe; J. E. K. Cooke
Source: Molecular Ecology Resources. doi: 10.1111/j.1755-0998.2009.02579.x
Publication Series: Scientific Journal (JRNL)
Station: Rocky Mountain Research Station
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DescriptionWe isolated 16 polymorphic microsatellite loci in the mountain pine beetle (Dendroctonus ponderosae Hopkins) and developed conditions for amplifying these markers in four multiplex reactions. Three to 14 alleles were detected per locus across two sampled populations. Observed and expected heterozygosities ranged from 0.000 to 0.902 and from 0.100 to 0.830, respectively. Three loci deviated from Hardy-Weinberg equilibrium in one sampled population. One of these loci may be sex linked. These markers will be useful in the study of population structure in this important pest species.
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CitationDavis, C. S.; Mock, K. E.; Bentz, B. J.; Bromilow, S. M.; Bartell, N. V.; Murray, B. W.; Roe, A. D.; Cooke, J. E. K. 2009. Isolation and characterization of 16 microsatellite loci in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae: Scolytinae). Molecular Ecology Resources. doi: 10.1111/j.1755-0998.2009.02579.x
KeywordsDendroctonus ponderosae, microsatellite, mountain pine beetle
- Landscape-scale genetic variation in a forest outbreak species, the mountain pine beetle (Dendroctonus ponderosae)
- Mountain pine beetle, Dendroctonus ponderosae (Coleoptera: Curculionidae, Scolytinae)
- Mountain pine beetle dynamics and reproductive success in post-fire lodgepole and ponderosa pine forests in northeastern Utah
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