Global and comparative protein profiles of the pronotum of the southern pine beetle, Dendroctonus frontalisAuthor(s): O. Pechanova; W.D. Stone; W. Monroe; T.E. Nebeker; K.D. Klepzig; C. Yuceer
Source: Insect Molecular Biology, Vol. 17(3): 261-277
Publication Series: Miscellaneous Publication
PDF: Download Publication (1.62 MB)
The southern pine beetle ( Dendroctonus frontalis Zimmermann) kills all pines within its range and is among the most important forest pest species in the US. Using a specialized mycangium surrounded by gland cells in the pronotum, adult females culture, transport, and inoculate two fungi into beetle galleries during oviposition. These fungal symbionts, to varying degrees, exclude antagonistic fungi and provide nutrients to larvae. However, the mechanisms (e.g. secreted antibiotic chemicals or nutrients, proteins or pathways) by which this relationship is maintained are not known. Here we present the first global and differential proteome profile of the southern pine beetle pronotum. Two-dimensional polyacrylamide electrophoresis, tandem mass spectrometry, and database searches revealed that the majority of pronotal proteins were related to energy-yielding metabolism, contractile apparati, cell structure, and defence. The identified proteins provide important insights into the molecular and biochemical processes of, and candidates for functional genomics to understand mycangia and pronotum functions in, the southern pine beetle.
- You may send email to email@example.com to request a hard copy of this publication.
- (Please specify exactly which publication you are requesting and your mailing address.)
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationPechanova, O.; Stone, W.D.; Monroe, W.; Nebeker, T.E.; Klepzig, K.D.; Yuceer, C. 2008. Global and comparative protein profiles of the pronotum of the southern pine beetle, Dendroctonus frontalis. Insect Molecular Biology, Vol. 17(3): 261-277
Keywordssouthern pine beetle, Dendroctonus frontalis, pronotum, mycangium, proteomics, differential gel electrophoresis.
- From Attack to Emergence: Interactions between Southern Pine Beetle, Mites, Microbes, and Trees
- Ultrastructure of the mycangium of the southern pine beetle, Dendroctonus frontalis (Coleoptera: Curculionidae, Scolytinae): complex morphology for complex interactions
- Competition between a biological control fungus, Ophiostoma piliferum, and symbionts of the southern pine beetle
XML: View XML