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    Author(s): Leland M. Humble
    Date: 2009
    Source: In: McManus, Katherine A; Gottschalk, Kurt W., eds. Proceedings. 20th U.S. Department of Agriculture interagency research forum on invasive species 2009; 2009 January 13-16; Annapolis, MD. Gen. Tech. Rep. NRS-P-51. Newtown Square, PA: U.S. Department of Agriculture, Forest Service, Northern Research Station: 78.
    Publication Series: Other
    Station: Northern Research Station
    PDF: View PDF  (119.13 KB)


    DNA barcoding is a molecular diagnostic tool that utilizes a standardized segment of the mitochondrial gene, cytochrome c oxidase subunit 1 (COI), for species identification. Attributes of the COI gene, including: the absence of introns; rarity of indels; ease of amplification by polymerase chain reaction; availability of universal primers; and ease of alignment after sequencing are of benefit for the development of diagnostic systems for arthropods. In order to identify unknown specimens, one must first create reference libraries of COI sequences from reliably identified voucher specimens.

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    Humble, Leland M. 2009. Building DNA barcode reference libraries for bark and woodborers

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