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    Author(s): Diego Martinez; Randy M. Berka; Bernard Henrissat; Markku Saloheimo; Mikko Arvas; Scott E. Baker; Jarod Chapman; Olga Chertkov; Pedro M. Coutinho; Daniel Cullen
    Date: 2008
    Source: Nature biotechnology. Vol. 26, no. 5: pages 553-560.
    Publication Series: Miscellaneous Publication
    PDF: View PDF  (382.53 KB)

    Description

    Trichoderma reesei is the main industrial source of cellulases and hemicellulases used to depolymerize biomass to simple sugars that are converted to chemical intermediates and biofuels, such as ethanol. We assembled 89 scaffolds (sets of ordered and oriented contigs) to generate 34 Mbp of nearly contiguous T. reesei genome sequence comprising 9,129 predicted gene models. Unexpectedly, considering the industrial utility and effectiveness of the carbohydrate-active enzymes of  T. reesei, its genome encodes fewer cellulases and hemicellulases than any other sequenced fungus able to hydrolyze plant cell wall polysaccharides. Many T. reesei genes encoding carbohydrate-active enzymes are distributed nonrandomly in clusters that lie between regions of synteny with other Sordariomycetes. Numerous genes encoding biosynthetic pathways for secondary metabolites may promote survival of T. reesei in its competitive soil habitat, but genome analysis provided little mechanistic insight into its extraordinary capacity for protein secretion. Our analysis, coupled with the genome sequence data, provides a roadmap for constructing enhanced T. reesei strains for industrial applications such as biofuel production.

    Publication Notes

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    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Martinez, Diego; Berka, Randy M.; Henrissat, Bernard; Saloheimo, Markku; Arvas, Mikko; Baker, Scott E.; Chapman, Jarod; Chertkov, Olga; Coutinho, Pedro M.; Cullen, Daniel, et al. 2008. Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn Hypocrea jecorina). Nature biotechnology. Vol. 26, no. 5: pages 553-560.

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    Keywords

    Cellulase, microbial metabolism, regulation, enzymes, industrial applications, biotechnology, gene expression, chemical engineering, alcohol, biodegradation, Trichoderma reesii, genetic engineering, nucleotide sequence, genomes, fungi, polysaccharides, biosynthesis, molecular genetics, Hypocrea jecorina, decay fungi, hemicellulose

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