Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): Konstantin V. Krutovsky; John Bradley St. Clair; Robert Saich; Valerie D. Hipkins; David B. Neale
    Date: 2009
    Source: Tree Genetics & Genomes. 5: 641-658
    Publication Series: Scientific Journal (JRNL)
    PDF: View PDF  (4.53 MB)

    Description

    Characterizing population structure using neutral markers is an important first step in association genetic studies in order to avoid false associations between phenotypes and genotypes that may arise from nonselective demographic factors. Population structure was studied in a wide sample of approximately 1,300 coastal Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco var. menziesii] trees from Washington and Oregon. This sample is being used for association mapping between cold hardiness and phenology phenotypes and single-nucleotide polymorphisms in adaptive-trait candidate genes. All trees were genotyped for 25 allozyme and six simple sequence repeat (SSR) markers using individual megagametophytes. Population structure analysis was done separately for allozyme and SSR markers, as well as for both datasets combined. The parameter of genetic differentiation was standardized to take into account high within-population variation in the SSR loci and to allow comparison with allozyme loci.

    Publication Notes

    • You may send email to pnw_pnwpubs@fs.fed.us to request a hard copy of this publication.
    • (Please specify exactly which publication you are requesting and your mailing address.)
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Krutovsky, Konstantin V.; St. Clair, John Bradley; Saich, Robert; Hipkins, Valerie D.; Neale, David B. 2009. Estimation of population structure in coastal Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco var. menziesii] using allozyme and microsatellite markers. Tree Genetics & Genomes. 5: 641-658.

    Cited

    Google Scholar

    Keywords

    Allozyme, genetic markers, microsatellites, population structure, Pseudotsuga menziesii, simple sequence repeats (SSR)

    Related Search


    XML: View XML
Show More
Show Fewer
Jump to Top of Page
https://www.fs.usda.gov/treesearch/pubs/34937