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    Author(s): Patricia L. JohnsonJane L. Hayes; John E. Rinehart; Walter S. Sheppard
    Date: 2008
    Source: The Canadian Entomologist
    Publication Series: Scientific Journal (JRNL)
    PDF: View PDF  (3.86 MB)

    Description

    Scolytus schevyrewi Semenov, the banded elm bark beetle, and S. multistriatus Marsham, the smaller European elm bark beetle, are morphologically similar. Reliance on adult external morphological characters for identification can be problematic because of wide within species variability and the need for good-quality specimens. The inability to identify developmental stages can also hamper early-detection programs. Using two character identification systems, genitalic (aedeagus) morphology, and DNA markers (random amplified polymorphic DNA polymerase chain reaction [RAPD-PCR]) to distinguish S. schevyrewi from S. multistriatus, we examined specimens from geographically distinct populations of both species collected from infested host trees or semiochemical-baited funnel traps. We found that aedeagus morphology can be used to identify the two species. The use of two oligonucleotide primers in the RAPD-PCR analysis yielded distinct DNA banding patterns for the two species. Species identification using RAPD-PCR analysis was validated by a blind test and used to make species identifications of larval specimens.

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    Citation

    Johnson, Patricia L.; Hayes, Jane L.; Rinehart, John E.; Sheppard, Walter S. 2008. Characterization of two non-native invasive bark beetles, Scolytus schevyrewi and Scolytus multistriatus (Coleoptera: Curculionidae: Scolytinae). The Canadian Entomologist. 140: 527-538.

    Keywords

    Character identification systems, genitalic (aedeagus) morphology, DNA markers, RAPD-PCR analysis

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