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    Author(s): Xiaomei Liu; Paula Pijut
    Date: 2010
    Source: Plant Cell Tissue and Organ Culture. 101: 49-57.
    Publication Series: Scientific Journal (JRNL)
    Station: Northern Research Station
    PDF: View PDF  (331.04 KB)

    Description

    A protocol for Agrobacterium-mediated transformation was developed for in vitro leaf explants of an elite, mature Prunus serotina tree. Agrobacterium tumefaciens strain EHA105 harboring an RNAi plasmid with the black cherry AGAMOUS (AG) gene was used. Bacteria were induced for 12 h with 200 µM acetosyringone for vir gene induction before leaf explant inoculation.

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    Citation

    Liu, Xiaomei; Pijut, Paula. 2010. Agrobacterium-medicated transformation of mature Prunus serotina (black cherry) and regeneration of trangenic shoots. Plant Cell Tissue and Organ Culture. 101: 49-57. DOI 10.1007/s11240-009-9662-3.

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    Keywords

    Agamous, genetic engineering, leaf explants, RNAi

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