Skip to Main Content
Identification and characterization of a protein kinase gene in the Lymantria dispar nultinucleocapsid nuclear polyhedrosis virusAuthor(s): David S. Bischoff; James M. Slavicek
Source: Journal of Virology. 68(3): 1728-1736.
Publication Series: Scientific Journal (JRNL)
Station: Northeastern Research Station
PDF: View PDF (2.07 MB)
DescriptionThe Lymantria dispar multinucleocapsid nuclear polyhedrosis virus (LdMNPV) gene encoding vPK has been cloned and sequenced. LdMNPV vPK shows a 24% amino acid identity to the catalytic domains of the eucaryotic protein kinases nPKC from rabbits, HSPKCE from humans, APLPKCB from Aplysia californica, and dPKC98F from Drosophila melanogaster, and homology to several other protein kinases from yeasts, mice, and bovines. The homology suggests that vPK is a serine/threonine protein kinase as defined by Hanks et al. (S.K. Hanks, A.M. Quinn, and T. Hunter, Science 241:42-52, 1988). Temporal expression studies indicate that vPK is expressed throughout the infection cycle beginning at 4 h postinfection, first as a delayed-early gene and subsequently as a late gene. Sequence analysis and primer extension reactions confirm the presence of distinct early and late transcription initiation regions. Expression of vPK with a rabbit reticulocyte system generated a 31-kDa protein, which is in close agreement with the predicted size of 32 kDa from the amino acid sequence. Phosphorylation activity of in vitro-expressed vPK was demonstrated by using calf thymus histones.
- Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat.
- During the capture process some typographical errors may occur.
- Please contact Sharon Hobrla, email@example.com if you notice any errors which make this publication unusable.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationBischoff, David S.; Slavicek, James M. 1994. Identification and characterization of a protein kinase gene in the Lymantria dispar Multinucleocapsid Nuclear Polyhedrosis Virus. Journal of Virology. 68(3): 1728-1736.
- Characterization of the Lymantria dispar nucleopolyhedrovirus 25K FP gene
- Identification and characterization of an early gene in the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus
- Molecular analysis of an enhancin gene in the Lymantria dispar nuclear polyhedrosis virus
XML: View XML