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    Author(s): James M. SlavicekNancy Hayes-Plazolles; Mary Ellen Kelly
    Date: 1995
    Source: Biological Control. 5: 251-261.
    Publication Series: Scientific Journal (JRNL)
    Station: Northeastern Research Station
    PDF: View PDF  (1.15 MB)

    Description

    Four genotypic variants of Lymantria dispar multimucleocapsid nuclear polyhedrosis virus (LdMNPV) were used to investigate the generation of few polyhedra (FP) mutants during viral propagation in the L. dispar 652Y cell line. Titers of budded virus, the percentage of infected cells producting polyhedra, the amount of polyhedra produced per cell, the proportion of virus exhibiting an FP phenotype, virion occlusion characteristics, and changes in genomic DNA digestion profiles were investigated through five serial passages.

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    Citation

    Slavicek, James M.; Hayes-Plazolles, Nancy; Kelly, Mary Ellen. 1995. Rapid formation of few polyhedral mutants of Lymantria dispar multinucleocapsid nuclear polyhedrosis virus during serial passage in cell culture. Biological Control. 5: 251-261.

    Keywords

    Lymantria dispar multinucleocapsid nuclear polyhedrosis virus, Lymantria dispar, gypsy moth, cell culture, few polyhedra mutants, biocontrol

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