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Rapid formation of few polyhedral mutants of Lymantria dispar multinucleocapsid nuclear polyhedrosis virus during serial passage in cell cultureAuthor(s): James M. Slavicek; Nancy Hayes-Plazolles; Mary Ellen Kelly
Source: Biological Control. 5: 251-261.
Publication Series: Scientific Journal (JRNL)
Station: Northeastern Research Station
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DescriptionFour genotypic variants of Lymantria dispar multimucleocapsid nuclear polyhedrosis virus (LdMNPV) were used to investigate the generation of few polyhedra (FP) mutants during viral propagation in the L. dispar 652Y cell line. Titers of budded virus, the percentage of infected cells producting polyhedra, the amount of polyhedra produced per cell, the proportion of virus exhibiting an FP phenotype, virion occlusion characteristics, and changes in genomic DNA digestion profiles were investigated through five serial passages.
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CitationSlavicek, James M.; Hayes-Plazolles, Nancy; Kelly, Mary Ellen. 1995. Rapid formation of few polyhedral mutants of Lymantria dispar multinucleocapsid nuclear polyhedrosis virus during serial passage in cell culture. Biological Control. 5: 251-261.
KeywordsLymantria dispar multinucleocapsid nuclear polyhedrosis virus, Lymantria dispar, gypsy moth, cell culture, few polyhedra mutants, biocontrol
- Identification of a Lymantria dispar nucleopolyhedrovirus isolate that does not accumulate few-polyhedra mutants during extended serial passage in cell culture
- Identification of a novel Lymantria dispar nucleopolyhedrovirus mutant that exhibits abnormal polyhedron formation and virion occlusion
- The Lymantria dispar nucleopolyhedrovirus contains the capsid-associated p24 protein gene
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