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Impact of deletion of the Lymantria dispar nucleopolyhedrovirus PEP gene on viral potency: expression of the green fluorescent protein prevents larval liquefactionAuthor(s): David S. Bischoff; James M. Slavicek
Source: Biological Control. 14: 51-59.
Publication Series: Scientific Journal (JRNL)
Station: Northeastern Research Station
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DescriptionThe Lymantria dispar multicapsid nucleopolyhedrovirus (LdMNPV) is an effective biological control agent of the gypsy moth, L. dispar, but is not in general use because the high cost of production limits availability. In an effort to generate a more cost efficient LdMNPV biopesticide, two recombinant baculoviruses were generated to evaluate the impact of deleting the polyhedral envelope protein (PEP) gene on viral potency. One of these baculoviruses had the PEP gene inactivated by insertional mutagenesis with the green fluorescent protein (GFP) gene from Aequorea victoria (vGFP: PEP-); and the other contained a partial deletion of the PEP gene from the LdMNPV viral genome (vPEP-). Both recombinant baculoviruses produced polyhedra that were lacking a polyhedral envelope and displayed an unusually pitted surface as analyzed by scanning and transmission electron microscopy.
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CitationBischoff, David S.; Slavicek, James M. 1999. Impact of deletion of the Lymantria dispar nucleopolyhedrovirus PEP gene on viral potency: expression of the green fluorescent protein prevents larval liquefaction. Biological Control. 14: 51-59.
Keywordsbaculovirus, Lymantria dispar nuclear polyhedrosis virus, Lymantria dispar, gypsy moth, polyhedral envelope protein
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