Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub


    The Lymantria dispar multicapsid nucleopolyhedrovirus (LdMNPV) is an effective biological control agent of the gypsy moth, L. dispar, but is not in general use because the high cost of production limits availability. In an effort to generate a more cost efficient LdMNPV biopesticide, two recombinant baculoviruses were generated to evaluate the impact of deleting the polyhedral envelope protein (PEP) gene on viral potency. One of these baculoviruses had the PEP gene inactivated by insertional mutagenesis with the green fluorescent protein (GFP) gene from Aequorea victoria (vGFP: PEP-); and the other contained a partial deletion of the PEP gene from the LdMNPV viral genome (vPEP-). Both recombinant baculoviruses produced polyhedra that were lacking a polyhedral envelope and displayed an unusually pitted surface as analyzed by scanning and transmission electron microscopy.

    Publication Notes

    • Check the Northern Research Station web site to request a printed copy of this publication.
    • Our on-line publications are scanned and captured using Adobe Acrobat.
    • During the capture process some typographical errors may occur.
    • Please contact Sharon Hobrla, if you notice any errors which make this publication unusable.
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.


    Bischoff, David S.; Slavicek, James M. 1999. Impact of deletion of the Lymantria dispar nucleopolyhedrovirus PEP gene on viral potency: expression of the green fluorescent protein prevents larval liquefaction. Biological Control. 14: 51-59.


    baculovirus, Lymantria dispar nuclear polyhedrosis virus, Lymantria dispar, gypsy moth, polyhedral envelope protein

    Related Search

    XML: View XML
Show More
Show Fewer
Jump to Top of Page