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Identification of a Lymantria dispar nucleopolyhedrovirus isolate that does not accumulate few-polyhedra mutants during extended serial passage in cell cultureAuthor(s): James M. Slavicek; Nancy Hayes-Plazolles; Mary Ellen Kelly
Source: Biological Control. 22: 159-168.
Publication Series: Scientific Journal (JRNL)
Station: Northeastern Research Station
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DescriptionDuring Lymantria dispar multinucleocapsid nucleopolyhedrovirus (LdMNPV) replication in cell culture, few-polyhedra (FP) mutants arise at a high frequency and become predominant after only a few serial passages. The formation of LdMNPV FP mutants was an impediment to successful production of polyhedra in cell culture bioreactors. We have isolated and characterized a strain of LdMNPV, termed 122b, that did not accumulate FP mutants after extended serial passage in cell culture. Wild-type virus strains exhibited decreased polyhedra production and increased budded virus (BV) release, and FP mutants were the predominant virus type present after only a few serial passages in L. dispar 652Y cells. In contrast, isolate 122b exhibited stable polyhedra and BV production through 14 serial passages.
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CitationSlavicek, James M.; Hayes-Plazolles, Nancy; Kelly, Mary Ellen. 2001. Identification of a Lymantria dispar nucleopolyhedrovirus isolate that does not accumulate few-polyhedra mutants during extended serial passage in cell culture. Biological Control. 22: 159-168.
Keywordsbaculovirus, Lymantria dispar nucleopolyhedrovirus, few-polyhedra mutant, cell culture, stable polyhedra production, Lymantria dispar, biological control
- Isolation of a baculovirus variant that exhibits enhanced polyhedra production stability during serial passage in cell culture
- Rapid formation of few polyhedral mutants of Lymantria dispar multinucleocapsid nuclear polyhedrosis virus during serial passage in cell culture
- Identification of a novel Lymantria dispar nucleopolyhedrovirus mutant that exhibits abnormal polyhedron formation and virion occlusion
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