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Identification and characterization of a RAPD-PCR marker for distinguishing Asian and North American gypsy mothsAuthor(s): K.J. Garner; J.M. Slavicek
Source: Insect Molecular Biology. 5(2): 81-91.
Publication Series: Scientific Journal (JRNL)
Station: Northeastern Research Station
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DescriptionThe recent introduction of the Asian gypsy moth (Lymantria dispar L.) into North America has necessitated the development of genetic markers to distinguish Asian moths from the established North American population, which originated in Europe. We used RAPD-PCR to identify a DNA length polymorphism that is diagnostic for the two moth strains. The polymorphism maps to an autosomal locus with codominant Mendelian inheritance. DNA sequence analyses of the Asian and North American forms enabled development of locus-specific primers so that this marker, designated FS-1, will be useful for strain identification under varying conditions in different laboratories.
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CitationGarner, K.J.; Slavicek, J.M. 1996. Identification and characterization of a RAPD-PCR marker for distinguishing Asian and North American gypsy moths. Insect Molecular Biology. 5(2): 81-91.
Keywordsgypsy moth, Lymantria dispar, RAPD-PCR, sequence-characterized amplified region (SCAR)
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- Pathogenicity of two nucleopolyhedrovirus products, Virin NSh and Gypchek, for Asian and North American gypsy moth larvae
- A comparison of the adaptations of strains of Lymantria dispar multiple nucleopolyhedrovirus to hosts from spatially isolated populations
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