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Properties of two Lymantria dispar nuclear polyhedrosis virus isolates obtained from the microbial pesticide GypchekAuthor(s): James M. Slavicek; John Podgwaite; Carita Lanner-Herrera
Source: Journal of Invertebrate Pathology. 59: 142-148.
Publication Series: Scientific Journal (JRNL)
Station: Northeastern Research Station
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DescriptionTwo Lymantria dispar nuclear polyhedrosis virus isolates, 5-6 and A2-1, differing in the phenotypic characteristic of the number of viral occlusions in infected cells, were obtained from a production lot of the microbial pesticide Gypchek and several of their replication properties were investigated and compared. Budded virus titer produced in cell culture, polyhedral inclusion body production in cell culture and in vivo, the number of virions present within occlusion body cross sections, and potency determinations suggest that isolate 5-6 is a few polyhedra plaque variant and that A2-1 is a many polyhedra wild-type isolate.
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CitationSlavicek, James M.; Podgwaite, John; Lanner-Herrera, Carita. 1992. Properties of two Lymantria dispar nuclear polyhedrosis virus isolates obtained from the microbial pesticide Gypchek. Journal of Invertebrate Pathology. 59: 142-148.
KeywordsLymantria dispar, nuclear polyhedrosis virus, plaque variant, few polyhedra mutant
- Rapid formation of few polyhedral mutants of Lymantria dispar multinucleocapsid nuclear polyhedrosis virus during serial passage in cell culture
- Identification of a Lymantria dispar nucleopolyhedrovirus isolate that does not accumulate few-polyhedra mutants during extended serial passage in cell culture
- Identification of a novel Lymantria dispar nucleopolyhedrovirus mutant that exhibits abnormal polyhedron formation and virion occlusion
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