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Comparative transcriptome and secretome analysis of wood decay fungi Postia placenta and Phanerochaete chrysosporiumAuthor(s): Amber J. Vanden Wymelenberg; Jill Gaskell; Michael Mozuch; Grzegorz Sabat; John Ralph; Oleksandr Skyba; Shawn D Mansfield; Robert A. Blanchette; Diego Martinez; Igor Grigoriev; Philip J Kersten; Daniel Cullen
Source: Applied and environmental microbiology. Vol. 76, no. 11 (June 2010): p. 3599-3610.
Publication Series: Miscellaneous Publication
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DescriptionCellulose degradation by brown rot fungi, such as Postia placenta, is poorly understood relative to the phylogenetically related white rot basidiomycete, Phanerochaete chrysosporium. To elucidate the number, structure, and regulation of genes involved in lignocellulosic cell wall attack, secretome and transcriptome analyses were performed on both wood decay fungi cultured for 5 days in media containing ball-milled aspen or glucose as the sole carbon source. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), a total of 67 and 79 proteins were identified in the extracellular fluids of P. placenta and P. chrysosporium cultures, respectively. Viewed together with transcript profiles, P. chrysosporium employs an array of extracellular glycosyl hydrolases to simultaneously attack cellulose and hemicelluloses. In contrast, under these same conditions, P. placenta secretes an array of hemicellulases but few potential cellulases. The two species display distinct expression patterns for oxidoreductase-encoding genes. In P. placenta, these patterns are consistent with an extracellular Fenton system and include the upregulation of genes involved in iron acquisition, in the synthesis of low-molecular-weight quinones, and possibly in redox cycling reactions.
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CitationVanden Wymelenberg, Amber J.; Gaskell, Jill; Mozuch, Michael; Sabat, Grzegorz; Ralph, John; Skyba, Oleksandr; Mansfield, Shawn D.; Blanchette, Robert A.; Martinez, Diego; Grigoriev, Igor; Kersten, Philip J; Cullen, Daniel. 2010. Comparative transcriptome and secretome analysis of wood decay fungi Postia placenta and Phanerochaete chrysosporium. Applied and Environmental Microbiology. 76(11): 3599-3610.
KeywordsLiquid chromatography, cellulose, biodegradation, mass spectrometry, enzymes, biotechnology, lignocellulose, Basidiomycetes, molecular genetics, fungi genetics, fungi, lignin, biodegradation, wood-decaying fungi, industrial applications, gene expression, genetic transcription, Postia placenta, Phanerochaete chrysosporium, brown rot, aspen, glucose, iron, quinone, chemical reactions, Populus, transcriptome, wood decay, secretome, white rot, decay fungi, Fenton system
- Significant alteration of gene expression in wood decay fungi Postia placenta and Phanerochaete chrysosporium by plant species
- Gene expression patterns of wood decay fungi Postia placenta and Phanerochaete chrysosporium are influenced by wood substrate composition during degradation
- Genome, transcriptome, and secretome analysis of wood decay fungus Postia placenta supports unique mechanisms of lignocellulose conversion
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