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    Author(s): Junyong Zhu; Wenyuan Zhu; Patricia OBryan; Bruce S. Dien; Shen Tian; Roland Gleisner; X.J. Pan
    Date: 2010
    Source: Applied microbiology and biotechnology. Vol. 86 (2010): p. 1355-1365.
    Publication Series: Miscellaneous Publication
    PDF: Download Publication  (317.38 KB)


    Lodgepole pine from forest thinnings is a potential feedstock for ethanol production. In this study, lodgepole pine was converted to ethanol with a yield of 276 L per metric ton of wood or 72% of theoretical yield. The lodgepole pine chips were directly subjected to sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) pretreatment and then disk-milled; the recovered cellulose substrate was quais-simultaneously saccharified enzymatically and fermented to ethanol using commercial cellulases and Saccharomyces cerevisiae D5A. The liquor stream from the pretreatment containing hydrolyzed sugars mainly from hemicelluloses was fermented by the same yeast strain after detoxification using an XAD resin column. The SPORL pretreatment was conducted at 180°C for a period of 25 min with a liquor-to-wood ratio of 3:1 (v/w) in a laboratory digester. Three levels of sulfuric acid charge (0.0%, 1.4%, and 2.2% on an oven dried wood basis in w/w) and three levels of sodium bisulfite charge (0.0%, 4.0%, and 8.0% in w/w) were applied. Mechanical and thermal energy consumption for milling and pretreatment were determined. These data were used to determine the efficiency of sugar recoveries and net ethanol energy production values and to formulate a preliminary mass and energy balance.

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    Zhu, Junyong; Zhu, Wenyuan; OBryan, Patricia; Dien, Bruce S.; Tian, Shen; Gleisner, Roland; Pan, X.J. 2010. Ethanol production from SPORL-pretreated lodgepole pine : preliminary evaluation of mass balance and process energy efficiency. Applied Microbiology and Biotechnology. 86: 1355-1365.


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    Cellulosic ethanol, SPORL, fermentation, saccharification, woody biomass, pretreatment, ethanol yield, alcohol, fuelwood, biomass energy, utilization, biotechnology, feedstock, energy consumption, sugars, lignocellulose, biodegradation, hemicellulose, cellulose, hydrolysis, lodgepole pine, sulfuric acid, enzymes, industrial applications, wood chips, yeast fungi, fungi, wood-decaying fungi, Saccharomyces cerevisiae, biomass fuel, ethanol, bioconversion, biorefining, wood extractives, chemical utilization, chips, sodium bisulfite

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