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    Author(s): Sarah F. Covert; Jennifer Bolduc; Daniel Cullen
    Date: 1992
    Source: Current genetics. Vol. 22 (1992): p. 407-413. Current genetics. Vol. 23 (1993): p. 374 (erratum).
    Publication Series: Miscellaneous Publication
    PDF: View PDF  (3.49 MB)

    Description

    Southern blot and nucleotide sequence analysis of Phanerochaete chrysosporium BKM-F-1767 genomic clones indicate that this wood-degrading fungus contains at least six genes with significant homology to the Trichoderma reesei cellobiohydrolase I gene (cbh1). Using pulsed-field gel electrophoresis to separate P. chrysosporium chromosomes, the six cellulase genes were found to hybridize to at least three different chromosomes, one of which is dimorphic. The organization of these genes was similar in another P. chrysosporium strain, ME 446. It is clear that, unlike T. reesei, the most well-studied cellulolytic fungus, P. chrysosporium contains a complex, cbh1-like gene family.

    Publication Notes

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    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Covert, Sarah F.; Bolduc, Jennifer; Cullen, Daniel 1992 Genomic organization of a cellulase gene family in Phanerochaete chrysosporium. Current genetics. Vol. 22 (1992): p. 407-413. Current genetics. Vol. 23 (1993): p. 374 (erratum).

    Keywords

    Molecular genetics, fungi, genetics, wood-decaying fungi, biodegradation, fungi, biotechnology, Basidiomycetes, nucleotide sequence, gene mapping, cellulase, genomes, cellulose 1, 4-beta-cellobiosidase, lignocellulose, gel electrophoresis, Phanerochaete chrysosporium, genetic engineering, wood decay, white rot, Trichoderma reesei, pulsed-field gel electrophoresis, southern blot analysis

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