Disrupting ecosystem components, while transferring and reconstructing them for experiments can produce myriad responses. Establishing the extent of these biological responses as the system approaches a new equilibrium allows us more reliably to emulate comparable native systems. That is, the sensitivity of analyzing ecosystem processes in a reconstructed system is improved by excluding the period when observed phenomena are primarily responses caused by establishing the experiment rather than effects of imposed treatments; achieved by determining the extent of any pulse of activity caused by preparatory procedures.
Rygiewicz, Paul T.; Monleon, Vicente J.; Ingham, Elaine R.; Martin, Kendall J.; Johnson, Mark G. 2010. Soil life in reconstructed ecosystems: initial soil food web responses after rebuilding a forest soil profile for a climate change experiment. Applied Soil Ecology. 45: 26-38.