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Transgenic manipulation of a single polyamine in poplar cells affects the accumulation of all amino acidsAuthor(s): Sridev Mohapatra; Rakesh Minocha; Stephanie Long; Subhash C. Minocha
Source: Amino Acids. 38: 1117-1129.
Publication Series: Scientific Journal (JRNL)
Station: Northern Research Station
PDF: Download Publication (572.45 KB)
DescriptionThe polyamine metabolic pathway is intricately connected to metabolism of several amino acids. While ornithine and arginine are direct precursors of putrescine, they themselves are synthesized from glutamate in multiple steps involving several enzymes. Additionally, glutamate is an amino group donor for several other amino acids and acts as a substrate for biosynthesis of proline and γ-aminobutyric acid, metabolites that play important roles in plant development and stress response. Suspension cultures of poplar (Populus nigra × maximowiczii), transformed with a constitutively expressing mouse ornithine decarboxylase gene, were used to study the effect of up-regulation of putrescine biosynthesis (and concomitantly its enhanced catabolism) on cellular contents of various protein and non-protein amino acids.
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CitationMohapatra, Sridev; Minocha, Rakesh; Long, Stephanie; Minocha, Subhash C. 2010. Transgenic manipulation of a single polyamine in poplar cells affects the accumulation of all amino acids. Amino Acids. 38: 1117-1129.
Keywordsgenetic manipulation, polyamines, ornithine decarboxylase, poplar
- Living with high putrescine: expression of ornithine and arginine biosynthetic pathway genes in high and low putrescine producing poplar cells
- Genetic manipulation of putrescine biosynthesis reprograms the cellular transcriptome and the metabolome
- Transgenic manipulation of the metabolism of polyamines in poplar cells
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