Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): Catherine Crosby; Lynne Carpenter-Boggs; Stewart Higgins; Nabil Khadduri
    Date: 2010
    Source: In: Riley, L. E.; Pinto, J. R.; Dumroese, R. K., tech. cords. National Proceedings: Forest and Conservation Nursery Associations-2009. Proc. RMRS-P-62. Fort Collins, CO: U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station. p. 32-34.
    Publication Series: Proceedings (P)
    Station: Rocky Mountain Research Station
    PDF: View PDF  (255 KB)

    Description

    Fusarium oxysporum and Cylindrocarpon destructans cause root disease that leads to significant crop losses in forest nurseries when not treated. Treatment currently relies on methyl bromide fumigation to eradicate soil pathogens. New environmental protection laws, however, are phasing out methyl bromide. Alternative chemical treatments are being tested, as well as biological fumigants such as seed meals and cover crops of Brassica spp. In this study, several different Brassica-based biofumigation treatments are being tested at Washington Department of Natural Resources Webster Forest Nursery in Olympia, WA. Fungal populations are being traced using molecular techniques such as PCR-ELISA and Real Time PCR. Use of molecular techniques to quantify the fungal pathogens should increase pathogen detection sensitivity and accuracy over the traditional dilution plating method.

    Publication Notes

    • You may send email to rmrspubrequest@fs.fed.us to request a hard copy of this publication.
    • (Please specify exactly which publication you are requesting and your mailing address.)
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Crosby, Catherine; Carpenter-Boggs, Lynne; Higgins, Stewart; Khadduri, Nabil. 2010. Detection and control of Fusarium oxysporum and Cylindrocarpon destructans in forest nursery soils. In: Riley, L. E.; Pinto, J. R.; Dumroese, R. K., tech. cords. National Proceedings: Forest and Conservation Nursery Associations-2009. Proc. RMRS-P-62. Fort Collins, CO: U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station. p. 32-34.

    Keywords

    Fusarium commune, Brassica spp., seed meals, biofumigation, PCR-ELISA, Real Time PCR

    Related Search


    XML: View XML
Show More
Show Fewer
Jump to Top of Page
https://www.fs.usda.gov/treesearch/pubs/37572