Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub


    This study demonstrated two in situ UV-vis spectrophotometric methods for rapid and temporally resolved measurements of cellulase adsorption onto cellulosic and lignocellulosic substrates during enzymatic hydrolysis. The cellulase protein absorption peak at 280 nm was used for quantification. The spectral interferences from light scattering by small fibers (fines) and particulates and from absorptions by lignin leached from lignocelluloses were corrected using a dual-wavelength technique. Wavelengths of 500 and 255 nm were used as secondary wavelengths for correcting spectral interferences from light scattering and absorption of leached lignin. Spectral interferences can also be eliminated by taking the second derivative of the measured spectra of enzymatic hydrolysate of cellulose or lignocelluloses. The in situ measured cellulase adsorptions in cellulose and lignocellulose suspensions by these two pectrophotometric methods showed general agreement with batch sampling assayed by the Bradford method. The in situ methods not only eliminated tedious batch sampling but also can resolve the kinetics of the initial adsorption process. The measured time-dependent cellulase adsorptions were found to follow pseudo-second-order kinetics.

    Publication Notes

    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.


    Liu, Hao; Zhu, J.Y.; Chai, X.S. 2011. In situ, rapid, and temporally resolved measurements of cellulase adsorption onto lignocellulosic substrates by UV-vis spectrophotometry. Langmuir. 27(1): 272-278.


    Google Scholar


    Cellulase, cellulose, lignocellulose, biodegradation, adsorption, hydrolysis, enzymes, biotechnology, industrial technology, absorption, lignin, spectrum analysis, spectrophotometry, suspensions, biomass energy, sugars, ultraviolet radiation, aspen, spruce, kinetics, biomass fuel, bioconversion, biorefining, chemical utilization, saccharification, endoglucanase, SPORL

    Related Search

    XML: View XML
Show More
Show Fewer
Jump to Top of Page