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    Author(s): Danilo Scordia; Salvatore L. Consentino; Thomas W. Jeffries
    Date: 2010
    Source: Bioresource technology. Vol. 101, no. 14 (July 2010): p. 5358–5365.
    Publication Series: Miscellaneous Publication
    PDF: Download Publication  (340.44 KB)


    Saccharum (Saccharum spontaneum L. ssp. aegyptiacum (Willd.) Hack.), is a rapidly growing, wide ranging high-yield perennial, suitable for second generation bioethanol production. This study evaluated oxalic acid as a pretreatment for bioconversion. Overall sugar yields, sugar degradation products, enzymatic glucan hydrolysis and ethanol production were studied as effects of temperature (150-190 °C), reaction time (10-40 min) and oxalic acid concentration (w/w). Time and temperature were combined into a: single parameter, Severity Factor (SF) [Log (Ro)], and related to oxalic acid using a response surface methodology Maximum total sugar yield was attained at a SF of 2.93 and 6.79% (w/w) oxalic acid, while maximum formation of sugar degradation products was observed at the highest SF (4.05) and 5% (w/w) oxalic acid. These were also the conditions for maximum simultaneous saccharification and fermentation (SSF) of the residual solids. Commercial cellulases and Saccharomyces cerevisiae attained 89.9% glucan conversion and 17.8 g/l ethanol. Pichia stipitis CBS 6054 fermented hemicellulosic hydrolysates from less severe conditions to ethanol with a yield of 0.35 (ge/gs). Maximal product yields were 69% of theoretical value and 90% of the SSF conversion efficiency for hydrolysate fermentation and SSF, respectively.

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    Scordia, Danilo; Consentino, Salvatore L.; Jeffries, Thomas W. 2010. Second generation bioethanol production from Saccharum spontaneum L. ssp. aegyptiacum (Willd.) Hack. Bioresource technology. Vol. 101, no. 14 (July 2010): p. 5358–5365.


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    Alcohol, oxalic acid, fermentation, Saccharum spontaneum, pretreatment, biomass utilization, biomass energy, fungi, biotechnology, yeast fungi, hemicellulose, temperature, industrial applications, Pichia stipitis, enzymes, hydrolysis, sugars, Saccharomyces cerevisiae, cellulose, cellulase, ethanol, saccharification, biomass fuel, biorefining, bioconversion, decay fungi, hydrolysates, biofuels, pretreatment, hemicellulose hydrolysate, simultaneous saccharification

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