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    Author(s): Grant T. Kirker; Susan V. Diehl; M. Lynn Prewitt
    Date: 2010
    Source: Proceedings, one hundred sixth annual meeting of the American Wood Protection Association ... Savannah, Georgia, May 23-25, 2010: volume 106. Birmingham, Ala. : American Wood Protection Association, c2010: p. 127-133.
    Publication Series: Miscellaneous Publication
    PDF: Download Publication  (195.94 KB)

    Description

    Currrent standards for soil-block testing have long been based on the effectiveness of preservative systems against only a small number of wood decay fungi and even fewer bacteria. Culture-independent molecular methods offer simple, reproducible means to obtain a more holistic view of the microbial communities that colonize wood throughout the decay process. By using a culture-independent PCR-based method called terminal restriction fragment length polymorphism (T-RFLP) analysis, we were able to detect shifts of fungal and bacterial communities in wood treated with sub-lethal concentrations of ACQ-C and CTN. T-RFLP takes into account all species of a taxonomic group and creates a community profile or “fingerprint,” where each peak in the profile represents a unique species. Both compounds appeared to change the patterns of bacterial succession completely, so that beginning and ending communities were significantly different in regard to species composition. Fungal species community structure was initially changed, but became more similar to untreated controls over time, presumably as the preservatives were depleted from samples. Subsequent depletion analysis found >60% depletion of preservatives from treated field stakes after 15 months exposure. Further modification to this process will eventually enable us to accurately identify fungal and bacterial species making up the microbial communities found in treated and decaying wood and offer new insights into the decay process.

    Publication Notes

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    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Kirker, Grant T.; Diehl, Susan V.; Prewitt, M. Lynn. 2010. The usual suspects: Fingerprinting microbial communities involved in decay of treated southern yellow pine. In: Proceedings, one hundred sixth annual meeting of the American Wood Protection Association. Savannah, GA, 2010 May 23-25: vol. 106. Birmingham, AL: American Wood Protection Association, c2010: 127-133.

    Keywords

    Biodegradation, wood deterioration, decay prevention, wood-decaying fungi, wood biodegradation, fungicides, wood preservatives, preserved wood, preservative testing, bacteria, antifungal agents, copper, fungal communities, microorganisms, microbial communities, BHT, preservatives, Butylated hydroxytoluene, BHT, chlorothalonil, field tests, exposure tests, decay fungi, wood decay, southern yellow pine, preservation, decay resistance, preservative treated wood, treated wood, biocides, ACQ, alkaline copper quaternary, wood decomposition, fungicidal properties, copper compounds, CTN, T-RFLP, stake tests, stake decay, microbial community analysis

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https://www.fs.usda.gov/treesearch/pubs/37934