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Identification of new polymorphic microsatellite markers in the NA1 and NA2 lineages of Phytophthora ramorumAuthor(s): A. Vercauteren; M. Larsen; E. Goss; N. Grunwald; M. Maes; K. Heungens
Publication Series: Scientific Journal (JRNL)
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DescriptionPhytophthora ramorum is a recently introduced pathogen in Europe and North America consisting of three clonal lineages. Due to the limited intralineage genetic variation, only a few polymorphic markers are available for use in studies involving the epidemiology and evolution of P. ramorum. A total of 159 primer pairs for candidate polymorphic SSR loci were tested with universal labeling. Four polymorphicmicrosatellite loci were identified within the NA1 lineage and one within the NA2 lineage, demonstrating the power and flexibility of the screening technique. The markers may significantly increase the number of genotypes that can be identified and as such can help better characterize the North American lineages of P. ramorum.
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CitationVercauteren, A.; Larsen, M.; Goss, E.; Grunwald, N.; Maes, M.; and Heungens, K. 2011. Identification of new polymorphic microsatellite markers in the NA1 and NA2 lineages of Phytophthora ramorum. Mycologia 103(6):1245–1249. DOI: 10.3852/10-420
Keywordsgenetic diversity, microsatellite markers, oomycetes, sudden oak death, universal labeling
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