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    Author(s): A. Vercauteren; M. Larsen; E. Goss; N. Grunwald; M. Maes; K. Heungens
    Date: 2011
    Source: Mycologia103(6):1245–1249
    Publication Series: Scientific Journal (JRNL)
    PDF: View PDF  (236.58 KB)

    Description

    Phytophthora ramorum is a recently introduced pathogen in Europe and North America consisting of three clonal lineages. Due to the limited intralineage genetic variation, only a few polymorphic markers are available for use in studies involving the epidemiology and evolution of P. ramorum. A total of 159 primer pairs for candidate polymorphic SSR loci were tested with universal labeling. Four polymorphicmicrosatellite loci were identified within the NA1 lineage and one within the NA2 lineage, demonstrating the power and flexibility of the screening technique. The markers may significantly increase the number of genotypes that can be identified and as such can help better characterize the North American lineages of P. ramorum.

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    Citation

    Vercauteren, A.; Larsen, M.; Goss, E.; Grunwald, N.; Maes, M.; and Heungens, K. 2011. Identification of new polymorphic microsatellite markers in the NA1 and NA2 lineages of Phytophthora ramorum. Mycologia 103(6):1245–1249. DOI: 10.3852/10-420

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    Keywords

    genetic diversity, microsatellite markers, oomycetes, sudden oak death, universal labeling

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