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Establishment and characterization of American elm cell suspension culturesAuthor(s): Steven M. Eshita; Joseph C. Kamalay; Vicki M. Gingas; Daniel A. Yaussy
Source: Plant Cell, Tissue and Organ Culture. 61: 245-249.
Publication Series: Scientific Journal (JRNL)
Station: Northern Research Station
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DescriptionCell suspension cultures of Dutch elm disease (DED)-tolerant and DED-susceptible American elms clones have been established and characterized as prerequisites for contrasts of cellular responses to pathogen-derived elicitors. Characteristics of cultured elm cell growth were monitored by A700 and media conductivity. Combined cell growth data for all experiments within a genotype showed relatively low variances and between-genotype contrasts during repeated passages showed no significant differences. Subculturing exponentially growing cells at 8-14 day intervals, within readily measured parameters of media conductivity (4.95-4.2 mmhos) and cell concentration (≥1.4 A700), consistently resulted in repeatable profiles of elm cell growth and minimized lag phase. Culture cells were essentially homogeneous after 5 subculture passages and their overall appearance was stable. We conclude that the described procedure resulted in consistent cultures suitable for elicitor treatment experiments.
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CitationEshita, Steven M.; Kamalay, Joseph C.; Gingas, Vicki M.; Yaussy, Daniel A. 2000. Establishment and characterization of American elm cell suspension cultures. Plant Cell, Tissue and Organ Culture. 61: 245-249.
Keywordscultured cell line, growth rates, tissue culture, Ulmus americana
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