Skip to Main Content
Isolations from the redbay ambrosia beetle, Xyleborus glabratus, confirm that the laurel wilt pathogen, Raffaelea lauricola, originated in AsiaAuthor(s): Thomas C. Harrington; Hye Young Yun; Sheng-Shan Lu; Hideaki Goto; Dilzara N. Aghayeva; Stephen W. Fraedrich
Source: Mycologia 103(5):1028-1036
Publication Series: Scientific Journal (JRNL)
Station: Southern Research Station
PDF: View PDF (93.25 KB)
DescriptionThe laurel wilt pathogen Raffaelea lauricola was hypothesized to have been introduced to the southeastern USA in the mycangium of the redbay ambrosia beetle, Xyleborus glabratus, which is native to Asia. To test this hypothesis adult X. glabratus were trapped in Taiwan and on Kyushu Island, Japan, in 2009, and dead beetles were sent to USA for isolation of fungal symbionts. Individual X. glabratus were macerated in glass tissue grinders, and the slurry was serially diluted and plated onto malt agar medium amended with cycloheximide, a medium semiselective for Ophiostoma species and their anamorphs, including members of Raffaelea. R. lauricola was isolated from 56 of 85 beetles in Taiwan and 10 of 16 beetles in Japan at up to an estimated 10 000 CFUs per beetle. The next most commonly isolated species was R. ellipticospora, which also has been recovered from X. glabratus trapped in the USA, as were two other fungi isolated from beetles in Taiwan, R. fusca and R. subfusca. Three unidentified Raffaelea spp. and three unidentified Ophiostoma spp. were isolated rarely from X. glabratus collected in Taiwan. Isolations from beetles similarly trapped in Georgia, USA, yielded R. lauricola and R. ellipticospora in numbers similar to those from beetles trapped in Taiwan and Japan. The results support the hypothesis that R. lauricola was introduced into the USA in mycangia of X. glabratus shipped to USA in solid wood packing material from Asia. However differences in the mycangial mycoflora of X. glabratus in Taiwan, Japan and USA suggest that the X. glabratus population established in USA originated in another part of Asia.
- You may send email to email@example.com to request a hard copy of this publication.
- (Please specify exactly which publication you are requesting and your mailing address.)
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationHarrington, Thomas C.; Yun, Hye Young; Lu, Sheng-Shan; Goto, Hideaki; Aghayeva, Dilzara N.; Fraedrich, Stephen W. 2011. Isolations from the redbay ambrosia beetle, Xyleborus glabratus, confirm that the laurel wilt pathogen, Raffaelea lauricola, originated in Asia. Mycologia 103(5):1028-1036.
KeywordsCinnamomum spp., Curculionidae, Lauraceae, mycangia, Ophiostoma spp., Persea spp., Raffaelea ellipticospora, R. fusca, R. subfusca, Scolytinae, vector
- Quantification of Propagules of the Laurel Wilt Fungus and Other Mycangial Fungi from the Redbay Ambrosia Beetle, Xyleborus glabratus
- New combinations in Raffaelea, Ambrosiella, and Hyalorhinocladiella, and four new species from the redbay ambrosia beetle, Xyleborus glabratus
- Emerging forest pest threat: Redbay ambrosia beetle and laurel wilt
XML: View XML