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    Author(s): G. Bucci; Thomas L. Kubisiak; W.L. Nance; P. Menozzi
    Date: 1997
    Source: Theoretical Applications of Genetics. 95:643-654.
    Publication Series: Miscellaneous Publication
    PDF: View PDF  (402 KB)

    Description

    The authors built a "consensus" partial linkage map based on RAPD markers using 48 sibships of eight megagametophytes each from a natural population of Norway spruce. A RAPD linkage map for a single individual from the same population had previously been constructed. Using 30 random decamers that had yielded 83 RAPD markers in the single-tree map, eight megagametophytes for each of the 48 sibships were screened. The linkage relationship among markers was estimated considering each family of eight megagametophytes as a progeny of a phase-unknown backcross mating between a heterozygous mother and a fictitious "recessive" father. Markers were assigned to windows using LOD = 2.0 and 2 = 0.4 as thresholds, and ordered using a criterion of interval support $ 2.0. For eight "windows" of recombination selected on the single-tree map, the scientists investigated the consistency of marker order in the two maps. They adopted restrictive criteria for rejecting co-linearity between the two locus orders. For each window the authors imposed the most likely locus order obtained from one data set to the other (and vice versa), obtaining two symmetrical log-likelihood differences. They considered the hypothesis of co-linearity rejected when both symmetrical differences were significant ()LOD > 3.0). By bootstrapping a subset of markers for each window (highly informative, "framework" loci chosen on the previous single-tree map using a matrix correlation method) the sampling variability of the single-tree and population maps was estimated. As expected, the population map was affected by a larger variability than the single-tree map. Heterogeneity in pairwise recombination fractions among groups of sibship revealed a (possible) alternative genomic arrangement detected within a single recombination window.

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    Citation

    Bucci, G.; Kubisiak, Thomas L.; Nance, W.L.; Menozzi, P. 1997. A population ''consensus'', partial linkage map of Picea abies Karst. based on RAPD markers. Theoretical Applications of Genetics. 95:643-654.

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