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    Description

    Nonspecific (nonproductive) binding (adsorption) of cellulase by lignin has been identified as a key barrier to reduce cellulase loading for economical sugar and biofuel production from lignocellulosic biomass. Sulfite Pretreatment to Overcome Recalcitrance of Lignocelluloses (SPORL) is a relatively new process, but demonstrated robust performance for sugar and biofuel production from woody biomass especially softwoods in terms of yields and energy efficiencies. This study demonstrated the role of lignin sulfonation in enhancing enzymatic saccharification of lignocelluloses – lignosulfonate from SPORL can improve enzymatic hydrolysis of lignocelluloses, contrary to the conventional belief that lignin inhibits enzymatic hydrolysis due to nonspecific binding of cellulase.

    Publication Notes

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    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Wang, ZJ; Lan, TQ; Zhu, JY. 2013. Lignosulfonate and elevated pH can enhance enzymatic saccharification of lignocelluloses. Biotechnology for Biofuels. 6(1): 10 p.

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    Keywords

    Enzymatic hydrolysis/saccharification, Lignin/lignosulfonate, Nonspecific/nonproductive binding/adsorption, Cellulase enzymes, Pretreatment

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https://www.fs.usda.gov/treesearch/pubs/42969