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Lignosulfonate and elevated pH can enhance enzymatic saccharification of lignocellulosesAuthor(s): ZJ Wang; TQ Lan; JY Zhu
Source: Biotechnology for Biofuels 2013, Volume 6, Number 1. 10 p.
Publication Series: Scientific Journal (JRNL)
Station: Forest Products Laboratory
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DescriptionNonspecific (nonproductive) binding (adsorption) of cellulase by lignin has been identified as a key barrier to reduce cellulase loading for economical sugar and biofuel production from lignocellulosic biomass. Sulfite Pretreatment to Overcome Recalcitrance of Lignocelluloses (SPORL) is a relatively new process, but demonstrated robust performance for sugar and biofuel production from woody biomass especially softwoods in terms of yields and energy efficiencies. This study demonstrated the role of lignin sulfonation in enhancing enzymatic saccharification of lignocelluloses – lignosulfonate from SPORL can improve enzymatic hydrolysis of lignocelluloses, contrary to the conventional belief that lignin inhibits enzymatic hydrolysis due to nonspecific binding of cellulase.
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CitationWang, ZJ; Lan, TQ; Zhu, JY. 2013. Lignosulfonate and elevated pH can enhance enzymatic saccharification of lignocelluloses. Biotechnology for Biofuels. 6(1): 10 p.
KeywordsEnzymatic hydrolysis/saccharification, Lignin/lignosulfonate, Nonspecific/nonproductive binding/adsorption, Cellulase enzymes, Pretreatment
- Lignosulfonate-mediated cellulase adsorption: enhanced enzymatic saccharification of lignocellulose through weakening nonproductive binding to lignin
- pH-Induced Lignin Surface Modification to Reduce Nonspecific Cellulase Binding and Enhance Enzymatic Saccharification of Lignocelluloses
- Bioconversion of woody biomass to biofuel and lignin co-product using sulfite pretreatment to overcome the recalcitrance of lignocelluloses (SPORL)
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