Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): Umesh P. AgarwalJ.Y. ZhuSally A. Ralph
    Date: 2013
    Source: Holzforschung 2013; Volume 67, Number 4: 2013; pp. 371–377.
    Publication Series: Scientific Journal (JRNL)
    Station: Forest Products Laboratory
    PDF: Download Publication  (792.17 KB)


    Hydrolysis experiments with commercial cellulases have been performed to understand the effects of cell wall crystallinity and lignin on the process. In the focus of the paper are loblolly pine wood samples, which were systematically delignified and partly ball-milled, and, for comparison, Whatman CC31 cellulose samples with different crystallinities. In pure cellulose samples, the percentage of cellulose hydrolysis was inversely proportional to the degree of crystallinity. For the loblolly pine samples, the extent of hydrolysis was low for the fraction with 74- to 149- µm particle size, but the ball-milled fraction was hydrolyzed easily. The impact of lignin removal was also influential as demonstrated on progressively delignified wood, i.e., the degree of saccharification increased with lignin removal. On the basis of data of 72 h hydrolysis time on materials with similar crystallinity, the cell wall was found to be eight times less hydrolyzable than Whatman CC31 cellulose. Taken together, cellulose crystallinity and composition are not as important as the ultrastructural changes caused by the disruption of the tightly packed regions of the cell wall that ensued upon acid chlorite delignification.

    Publication Notes

    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.


    Agarwal, Umesh P.; Zhu, J.Y.; Ralph, Sally A. 2013. Enzymatic hydrolysis of loblolly pine: effects of cellulose crystallinity and delignification. Holzforschung. 67(4): 371–377.


    Google Scholar


    ball milling, cellulase, cellulose, crystallinity, enzymatic hydrolysis, hydrolysis, lignin, loblolly pine, wood

    Related Search

    XML: View XML
Show More
Show Fewer
Jump to Top of Page