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Polyamine and ethylene biosynthesis in relation to somatic embryogenesis in carrot (Daucus carota L.) cell culturesAuthor(s): Subhash C. Minocha; Cheryl A. Robie; Akhtar J. Khan; Nancy S. Papa; Andrew I. Samuelsen; Rakesh Minocha
Source: In: Flores H.E.; Arteca R.N.; Shannon J.C., eds. Polyamines and ethylene: biochemistry, physiology and interactions. Rockville, MD: American Society of Plant Physiologists: 339-342.
Publication Series: Paper (invited, offered, keynote)
Station: Northern Research Station
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DescriptionCarrot cell cultures provide a model experimental system for the analysis of biochemical and molecular events associated with morphogenesis in plants (3, 4, 5, 14). Among the biochemical changes accompanying somatic embryogenesis in this tissue is an increased biosynthesis ofpolyamines (1, 2, 7, 10, 11, 13). A variety of inhibitors of polyamine biosynthetic enzymes have been used to analyze the role of polyamines in somatic embryogenesis. A summary of our work on the effects of DFMO, DFMA, MGBG and CHAP on somatic embryogenesis, cellular polyamine levels, and activities of ADC, ODC, AdoMet decarboxylase, and AdoMet-synthetase in carrot cell cultures is reported here. Details of materials and methods and results are published elsewhere (6, 8, 9, 12, 13). The results obtained so far support our working hypothesis (7,13) that (a) ethylene is a major suppressor of embryogenesis, and its production is promoted by auxin; and (b) promotion of polyamine biosynthesis through increased utilization of S-adenosylmethionine may adversely affect ACC and ethylene syntheses, which could, in turn, promote somatic embryogenesis.
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CitationMinocha, Subhash C.; Robie, Cheryl A.; Khan, Akhtar J.; Papa, Nancy S.; Samuelsen, Andrew I.; Minocha, Rakesh. 1990. Polyamine and ethylene biosynthesis in relation to somatic embryogenesis in carrot (Daucus carota L.) cell cultures. In: Flores H.E.; Arteca R.N.; Shannon J.C., eds. Polyamines and ethylene: biochemistry, physiology and interactions. Rockville, MD: American Society of Plant Physiologists: 339-342.
- Assays for the activities of polyamine biosynthetic enzymes using intact tissues
- Effects of polyamine biosynthesis inhibitors on S-adenosylmethionine synthetase and S-adenosylmethionine decarboxylase activities in carrot cell cultures
- Role of polyamines in DNA synthesis of Catharanthus roseus cells grown in suspension culture
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