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Comparison of in situ and in vitro baiting assays for Phytophthora ramorum survey of waterways in the southeastern United StatesAuthor(s): Steven Oak; Jaesoon Hwang; Steven Jeffers
Source: In: Frankel, S.J.; Kliejunas, J.T.; Palmieri, K.M.; Alexander, J.M. tech. coords. Proceedings of the sudden oak death fifth science symposium. Gen. Tech. Rep. PSW-GTR-243. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: 51-52
Publication Series: General Technical Report (GTR)
Station: Pacific Southwest Research Station
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DescriptionIn situ baiting with whole, intact leaves of Rhododendron spp. has been employed since 2006 by the National Phytophthora ramorum Early Detection survey of forests (national survey). Using this method, P. ramorum was detected for the first time in national survey waterways draining 12 infested ornamental crop nurseries in Alabama, Florida, Georgia, Mississippi, North Carolina, and Washington as well as many forest areas in California and Oregon. In situ baiting periods lasting 1 to 3 weeks allow sampling large volumes of water over time, but also can result in loss of bait leaves from storm surges and vandalism. In situ baiting also requires two site visits for a single bait set (once to deploy and once to retrieve) and sustained water flow. An in vitro assay without these limitations was evaluated in experimental applications, and it has been effective at recovering P. ramorum. Therefore, we used both the in situ and in vitro baiting assays simultaneously for the 2011 National Survey for 12 P. ramorum-infested waterways in five states in the southeastern United States to compare relative performance under field conditions.
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CitationOak, Steven; Hwang, Jaesoon; Jeffers, Steven. 2013. Comparison of in situ and in vitro baiting assays for Phytophthora ramorum survey of waterways in the southeastern United States. In: Frankel, S.J.; Kliejunas, J.T.; Palmieri, K.M.; Alexander, J.M. tech. coords. Proceedings of the sudden oak death fifth science symposium. Gen. Tech. Rep. PSW-GTR-243. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: 51-52.
KeywordsSudden oak death, Phytophthora ramorum, invasive species, tanoak, Notholithocarpus densiflorus, coast live oak, Quercus agrifolia, Japanese larch, Larix kaempferi
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