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    Author(s): Joyce Eberhart; Elilzabeth Stamm; Jennifer Parke
    Date: 2013
    Source: In: Frankel, S.J.; Kliejunas, J.T.; Palmieri, K.M.; Alexander, J.M. tech. coords. Proceedings of the sudden oak death fifth science symposium. Gen. Tech. Rep. PSW-GTR-243. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: 130-133
    Publication Series: General Technical Report (GTR)
    Station: Pacific Southwest Research Station
    PDF: View PDF  (0 B)

    Description

    Germination of chlamydospores is difficult to accurately assess when chlamydospores are attached to remnants of supporting hyphae. We developed two approaches for closely observing and rigorously quantifying the frequency of chlamydospore germination in vitro. The plate marking and scanning method was useful for quantifying germination of large numbers of chlamydospores over a 7-day period. A method involving time lapse photography of microscope slide cultures was effective for visualizing the process of germination over shorter time periods.

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    Citation

    Eberhart, Joyce; Stamm, Elilzabeth; Parke, Jennifer. 2013. Methods for assessing Phytophthora ramorum chlamydospore germination. In: Frankel, S.J.; Kliejunas, J.T.; Palmieri, K.M.; Alexander, J.M. tech. coords. Proceedings of the sudden oak death fifth science symposium. Gen. Tech. Rep. PSW-GTR-243. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: 130-133.

    Keywords

    Phytophthora ramorum, chlamydospore, germination, assessment, banana slug

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