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    Author(s): Mingie Jin; Cory Sarks; Christa Gunawan; Benjamin D. Bice; Shane P. Simonett; Ragothaman Avanasi Narasimhan; Laura B. Willis; Bruce E. Dale; Venkatesh Balan; Trey K. Sato
    Date: 2013
    Source: Biotechnology for Biofuels, Volume 6, Number 108, 2013; 14 p.
    Publication Series: Scientific Journal (JRNL)
    Station: Forest Products Laboratory
    PDF: Download Publication  (669.6 KB)


    Simultaneous saccharification and co-fermentation (SSCF) process involves enzymatic hydrolysis of pretreated lignocellulosic biomass and fermentation of glucose and xylose in one bioreactor. The optimal temperatures for enzymatic hydrolysis are higher than the standard fermentation temperature of ethanologenic Saccharomyces cerevisiae. Moreover, degradation products resulting from biomass pretreatment impair fermentation of sugars, especially xylose, and can synergize with high temperature stress. One approach to resolve both concerns is to utilize a strain background with innate tolerance to both elevated temperatures and degradation products.

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    Jin, Mingie; Sarks, Cory; Gunawan, Christa; Bice, Benjamin D.; Simonett, Shane P.; Narasimhan, Ragothaman Avanasi; Willis, Laura B.; Dale, Bruce E.; Balan, Venkatesh; Sato, Trey K. 2013. Phenotypic selection of a wild Saccharomyces cerevisiae strain for simultaneous saccharification and co-fermentation of AFEX(TM) pretreated corn stover. Biotechnology for Biofuels. 6(108): 14 p.


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    Thermo-tolerance, Xylose fermentation, S. cerevisiae, SSCF, AFEX, Degradation products, Ethanol

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