Skip to Main Content
High diverstiy and widespread occurrence of mitotic spore mats in ectomycorrhizal PezizalesAuthor(s): R.A. Healy; M.E. Smith; G.M. Bonito; D.H. Pfister; Z.-W. Ge; G.G. Guevara; G. Williams; K. Stafford; L. Kumar; T. Lee; C. Hobart; J. Trappe; R. Vilgalys; D.J. McLaughlin
Source: Molecular Ecology. 22(6):1717-1732
Publication Series: Scientific Journal (JRNL)
Station: Pacific Northwest Research Station
Download Publication (1.56 MB)
DescriptionFungal mitospores may function as dispersal units and/ or spermatia and thus play a role in distribution and/or mating of species that produce them. Mitospore production in ectomycorrhizal (EcM) Pezizales is rarely reported, but here we document mitospore production by a high diversity of EcM Pezizales on three continents, in both hemispheres. We sequenced the internal transcribed spacer (ITS) and partial large subunit (LSU) nuclear rDNA from 292 spore mats (visible mitospore clumps) collected in Argentina, Chile, China, Mexico and the USA between 2009 and 2012. We collated spore mat ITS sequences with 105 fruit body and 47 EcM root sequences to generate operational taxonomic units (OTUs). Phylogenetic inferences were made through analyses of both molecular data sets. A total of 48 OTUs from spore mats represented six independent EcM Pezizales lineages and included truffles and cup fungi. Three clades of seven OTUs have no known meiospore stage. Mitospores failed to germinate on sterile media, or form ectomycorrhizas on Quercus, Pinus and Populus seedlings, consistent with a hypothesized role of spermatia. The broad geographic range, high frequency and phylogenetic diversity of spore mats produced by EcM Pezizales suggests that a mitospore stage is important for many species in this group in terms of mating, reproduction and/or dispersal.
- You may send email to email@example.com to request a hard copy of this publication.
- (Please specify exactly which publication you are requesting and your mailing address.)
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationHealy, R.A.; Smith, M.E.; Bonito, G.M.; Pfister, D.H.; Ge, Z.-W.; Guevara, G.G.; Williams, G.; Stafford, K.; Kumar, L.; Lee, T.; Hobart, C.; Trappe, J.; Vilgalys, R.; McLaughlin, D.J. 2013. High diverstiy and widespread occurrence of mitotic spore mats in ectomycorrhizal Pezizales. Molecular Ecology. 22(6):1717-1732.
Keywordscryptic diversity, ectomycorrhizal Pezizales, environmental sequencing, mitospore, truffle
- The vertical development of early successional forests in northern Michigan, USA
- Restoration of bottomland hardwood forest across a treatment intensity gradient.
- Restoration of bottomland hardwood forests across a treatment intensity gradient
XML: View XML