Skip to Main Content
Spirostaphylotrichin W, a spirocyclic γ-lactam isolated from liquid culture of Pyrenophora semeniperda, a potential mycoherbicide for cheatgrass (Bromus tectorum) biocontrolAuthor(s): Marco Masia; Susan Meyer; Suzette Clement; Anna Andolfi; Alessio Cimmino; Antonio Evidente
Source: Tetrahedron. 70(7): 1497-1501.
Publication Series: Scientific Journal (JRNL)
Station: Rocky Mountain Research Station
Download Publication (414.05 KB)
Related Research Highlights
Cheatgrass Biocontrol with "Black Fingers of Death"
DescriptionA novel spirocyclic γ-lactam, named spirostaphylotrichin W (1), was isolated together with the well known and closely related spirostaphylotrichins A, C, D, R and V, as well as triticone E, from the liquid cultures of Pyrenophora semeniperda (anamorph: Drechslera), a seed pathogen proposed for cheatgrass (Bromus tectorum) biocontrol. Spirostaphylotrichin W was characterized as (3S*,4S*,5S*,6S*,9Z,10Z)-4,6-dihydroxy-2,3-dimethoxy-3-methyl-10-propyliden-2-azaspiro [4.5]dec-8-ene-1,7-dione, by spectroscopic and chemical methods. The relative stereochemistry of spirostaphylotrichin W was assigned using NOESY experiments and in comparison to those of spirostaphylotrichin V (2) and triticone E (6). In fact, the relative stereochemistry at C-3 was the same of that of 2, while that at C-4 and C-6 was inverted in respect to that reported, respectively, for 2 and 6. In a B. tectorum coleoptile bioassay at concentration of 10-3, spirostaphylotrichin A proved to be the most active compound, followed by spirostaphylotrichins C and D. Spirostaphylotrichin W and V showed mild toxicity while spirostaphylotrichin R and triticone E were not active. When tested on host and non-host plants by leaf puncture bioassay, spirostaphylotrichins A, C and D caused the appearance of necrotic spots while the other compounds were inactive.
- You may send email to firstname.lastname@example.org to request a hard copy of this publication.
- (Please specify exactly which publication you are requesting and your mailing address.)
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationMasia, Marco; Meyer, Susan; Clement, Suzette; Andolfi, Anna; Cimmino, Alessio; Evidente, Antonio. 2014. Spirostaphylotrichin W, a spirocyclic gamma-lactam isolated from liquid culture of Pyrenophora semeniperda, a potential mycoherbicide for cheatgrass (Bromus tectorum) biocontrol. Tetrahedron. 70(7): 1497-1501.
KeywordsBromus tectorum, biocontrol, Pyrenophora semeniperda, Spirocyclic γ-lactam, Spirostaphylotrichin W
- Phytotoxic activity of metabolites isolated from Rutstroemia sp.n., the causal agent of bleach blonde syndrome on cheatgrass (Bromus tectorum)
- Fire effects on the mobilization and uptake of nitrogen by cheatgrass (Bromus tectorum L.)
- Is Pyrenophora semeniperda the cause of downy brome (Bromus tectorum) die-offs?
XML: View XML