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    The effects of lignosulfonate (LS) on enzymatic saccharification of pure cellulose were studied. Four fractions of LS with different molecular weight (MW) prepared by ultrafiltration of a commercial LS were applied at different loadings to enzymatic hydrolysis of Whatman paper under different pH. Using LS fractions with low MW and high degree of sulfonation can enhance enzymatic cellulose saccharification despite LS can bind to cellulase nonproductively. The enhancing effect varies with LS properties, its loading, and hydrolysis pH. Inhibitive effect on cellulose saccharification was also observed using LS with large MW and low degree of sulfonation. The concept of “LS-cellulase aggregate stabilized and enhanced cellulase binding” was proposed to explain the observed enhancement of cellulose saccharification. The concept was demonstrated by the linear correlation between the measured amount of bound cellulase and saccharification efficiency with and without LS of different MW in a range of pH.

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    Lou, Hongming; Zhou, Haifeng; Li, Xiuli; Wang, Mengxia; Zhu, J.Y.; Qiu, Xueqing. 2014. Understanding the effects of lignosulfonate on enzymatic saccharification of pure cellulose. Cellulose, Volume 21, 2014; pp. 1351-1359.


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    enzymatic hydrolysis, cellulose saccharification, lignosulfonate, surfactant cellulase binding, hydrophobic interaction, nonproductive binding

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