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Influence of Populus Genotype on Gene Expression by the Wood Decay Fungus Phanerochaete chrysosporiumAuthor(s): Jill Gaskell; Amber Marty; Michael Mozuch; Philip J. Kersten; Sandra Splinter Bondurant; Grzegorz Sabat; Ali Azarpira; John Ralph; Oleksandr Skyba; Shawn D. Mansfield; Robert A. Blanchette; Dan Cullen
Source: Appl. Environ. Microbiol. 2014, Volume 80, Number 18, 2014; pp. 5828-5835.
Publication Series: Scientific Journal (JRNL)
Station: Forest Products Laboratory
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DescriptionWe examined gene expression patterns in the lignin-degrading fungus Phanerochaete chrysosporium when it colonizes hybrid poplar (Populus alba tremula) and syringyl (S)-rich transgenic derivatives. Acombination ofmicroarrays and liquid chromatography- tandem mass spectrometry (LC-MS/MS) allowed detection of a total of 9,959 transcripts and 793 proteins. Comparisons of P. chrysosporium transcript abundance in medium containing poplar or glucose as a sole carbon source showed 113 regulated genes, 11 of which were significantly higher (> 2-fold, P < 0.05) in transgenic line 64 relative to the parental line. Possibly related to the very large amounts of syringyl (S) units in this transgenic tree (94mol% S), several oxidoreductases were among the upregulated genes. Peptides corresponding to a total of 18 oxidoreductases were identified in medium consisting of biomass from line 64 or 82 (85 mol% S) but not in the parental clone (65 mol% S). These results demonstrate that P. chrysosporium gene expression patterns are substantially influenced by lignin composition.
CitationGaskell, Jill; Marty, Amber; Mozuch, Michael; Kersten, Philip J.; Bondurant, Sandra Splinter; Sabat, Grzegorz; Azarpira, Ali; Ralph, John; Skyba, Oleksandr; Mansfield, Shawn D.; Blanchette, Robert A.; Cullen, Dan. 2014. Influence of Populus Genotype on Gene Expression by the Wood Decay Fungus Phanerochaete chrysosporium. Appl. Environ. Microbiol. 2014, Volume 80, Number 18, 2014; pp. 5828-5835.
KeywordsPhanerochaete, lignin, Populus, transgenic, transcriptome, secretome
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