Skip to Main Content
Agrobacterium-mediated transformation of black cherry for flowering control and insect resistanceAuthor(s): Ying Wang; Paula M. Pijut
Source: Plant Cell, Tissue and Organ Culture. 119(1): 107-116.
Publication Series: Scientific Journal (JRNL)
Station: Northern Research Station
View PDF (1.33 MB)
DescriptionBlack cherry is one of the most valuable hardwood species for cabinetry, furniture, and veneer. The goal of this study was to develop transgenic black cherry plants with reproductive sterility and enhanced insect resistance. Black cherry TERMINAL FLOWER 1 (PsTFL1) was overexpressed under the control of the CaMV 35S promoter in black cherry via Agrobacterium-mediated transformation, as a strategy for gene containment. PsTFL1 is a homolog of Arabidopsis TFL1 which is known to play a key role in regulating flowering time by counteracting with FLOWERING LOCUS T and repressing the transcription of the floral-related genes. The elevated expression level of PsTFL1 was proven to be able to significantly delay flowering and cause abnormal floral structure which led to sterility in Arabidopsis. Therefore, the overexpression of PsTFL1 was expected to induce the similar phenotype in black cherry to achieve reproductive sterility.
- Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat.
- During the capture process some typographical errors may occur.
- Please contact Sharon Hobrla, email@example.com if you notice any errors which make this publication unusable.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationWang, Ying; Pijut, Paula M. 2014. Agrobacterium-mediated transformation of black cherry for flowering control and insect resistance. Plant Cell, Tissue and Organ Culture. 119(1): 107-116.
KeywordsCyanogenesis, Flowering control, Gene silencing, Genetic transformation, Insect resistance, Prunus serotina
- Cloning and characterization of prunus serotina AGAMOUS, a putative flower homeotic gene
- The Spotted Wing Drosophila, Drosophila suzukii (Diptera: Drosophilidae): A New Pest of Concern for Black Cherry, Prunus serotina, on the High Allegheny Plateau in Pennsylvania
- Isolation and characterization of a TERMINAL FLOWER 1 homolog from Prunus serotina Ehrh
XML: View XML