Skip to Main Content
Production of cellulose nanofibrils from bleached eucalyptus fibers by hyperthermostable endoglucanase treatment and subsequent microfluidizationAuthor(s): Wangxia Wang; Michael D. Mozuch; Ronald C. Sabo; Phil Kersten; J.Y. Zhu; Yongcan Jin
Source: Cellulose 22, 2015; pp. 351-361.
Publication Series: Scientific Journal (JRNL)
Station: Forest Products Laboratory
Download Publication (1.63 MB)
DescriptionA GH5 hyperthermostable endoglucanase from the archaeon Pyrococcus honkoshii (ph-GH5) and a commercial endoglucanase FR were used to treat bleached eucalyptus pulp (BEP) fibers to produce cellulose nanofibrils (CNFs) through subsequent microfluidization Enzymatic treatments facilitated CNF production due to the reduced degree of polymerization (DP) of the fibers SEM imaging indicated that FR reduced fiber DP drastically and resulted in much shorter fibers than with Ph-GH5, even at very low dosages (1 mg protein/g fiber) of FR treatment compared with a high dosage (10 mg protein/g fiber) of Ph-GH5. The fibers treated with FR were much more uniform in length perhaps due to the presence of exoglucanase and beta-glucosidase saccharifying short microfibers into glucose. TEM imaging indicated that Ph-GH5 produced longer and entangled CNFs than FR with the same number of microfluidization passes. However, the CNF diameters were approximately the same for all CNFs from enzyme-treated fibers using both endoglucanases at two dosages (1 or 10 mg protein/g fiber). CNFs produced from BEP fibers without enzymatic treatment showed larger diameters than those with enzymatic treatment.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationWang, Wangxia; Mozuch, Michael D.; Sabo, Ronald C.; Kersten, Phil; Zhu, J.Y.; Jin, Yongcan. 2015. Production of cellulose nanofibrils from bleached eucalyptus fibers by hyperthermostable endoglucanase treatment and subsequent microfluidization. Cellulose 22, 2015; pp. 351-361.
KeywordsHyperthermophilic endoglucanase, pretreatment, microfluidization, nanofibrillated cellulose
- Physical and Mechanical Properties of Cellulose Nanofibril Films from Bleached Eucalyptus Pulp by Endoglucanase Treatment and Microfluidization
- Endoglucanase post-milling treatment for producing cellulose nanofibers from bleached eucalyptus fibers by a supermasscolloider
- Study on the Modification of Bleached Eucalyptus Kraft Pulp Using Birch Xylan
XML: View XML