Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): Wangxia Wang; Michael D. MozuchRonald C. SaboPhil KerstenJ.Y. Zhu; Yongcan Jin
    Date: 2015
    Source: Cellulose 22, 2015; pp. 351-361.
    Publication Series: Scientific Journal (JRNL)
    Station: Forest Products Laboratory
    PDF: View PDF  (1.63 MB)

    Description

    A GH5 hyperthermostable endoglucanase from the archaeon Pyrococcus honkoshii (ph-GH5) and a commercial endoglucanase FR were used to treat bleached eucalyptus pulp (BEP) fibers to produce cellulose nanofibrils (CNFs) through subsequent microfluidization Enzymatic treatments facilitated CNF production due to the reduced degree of polymerization (DP) of the fibers SEM imaging indicated that FR reduced fiber DP drastically and resulted in much shorter fibers than with Ph-GH5, even at very low dosages (1 mg protein/g fiber) of FR treatment compared with a high dosage (10 mg protein/g fiber) of Ph-GH5. The fibers treated with FR were much more uniform in length perhaps due to the presence of exoglucanase and beta-glucosidase saccharifying short microfibers into glucose. TEM imaging indicated that Ph-GH5 produced longer and entangled CNFs than FR with the same number of microfluidization passes. However, the CNF diameters were approximately the same for all CNFs from enzyme-treated fibers using both endoglucanases at two dosages (1 or 10 mg protein/g fiber). CNFs produced from BEP fibers without enzymatic treatment showed larger diameters than those with enzymatic treatment.

    Publication Notes

    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Wang, Wangxia; Mozuch, Michael D.; Sabo, Ronald C.; Kersten, Phil; Zhu, J.Y.; Jin, Yongcan. 2015. Production of cellulose nanofibrils from bleached eucalyptus fibers by hyperthermostable endoglucanase treatment and subsequent microfluidization. Cellulose 22, 2015; pp. 351-361.

    Cited

    Google Scholar

    Keywords

    Hyperthermophilic endoglucanase, pretreatment, microfluidization, nanofibrillated cellulose

    Related Search


    XML: View XML
Show More
Show Fewer
Jump to Top of Page
https://www.fs.usda.gov/treesearch/pubs/47688