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Mapping fusiform rust resistance genes within a complex mating design of loblolly pineAuthor(s): Tania Quesada; Marcio F.R. Resende Jr.; Patricio Munoz; Jill L. Wegrzyn; David B. Neale; Matias Kirst; Gary F. Peter; Salvador A. Gezan; C.Dana Nelson; John M. Davis
Source: Forests 2014, 5, 347-362
Publication Series: Scientific Journal (JRNL)
Station: Southern Research Station
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DescriptionFusiform rust resistance can involve gene-for-gene interactions where resistance (Fr) genes in the host interact with corresponding avirulence genes in the pathogen, Cronartium quercuum f.sp. fusiforme (Cqf). Here, we identify trees with Fr genes in a loblolly pine population derived from a complex mating design challenged with two Cqf inocula (one gall and 10 gall mixtures). We used single nucleotide polymorphism (SNP) genotypes at sufficient density to ensure linkage between segregating markers and Fr genes identifying SNPs that explained high proportions of variance in disease incidence using BayesC, that also were significant using Bayesian Association with Missing Data (BAMD) software. Two SNPs mapped near Fr1 and generated significant LOD scores in single marker regression analyses for Fr1/fr1 parent 17 as well as four other parents. One SNP mapped near Fr8 and was significant for parent 28. Two SNPs mapped to linkage groups not previously shown to contain Fr genes and were significant for three parents. Parent 2 showed evidence of Fr gene stacking. Our results suggest that it is feasible to identify trees segregating for Fr genes, and to map Fr genes, based on parental analysis of SNPs that cosegregate with disease incidence in designed resistance screening trials.
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CitationQuesada, Tania;Resende Jr., Marcio F.R.; Munoz, Patricio; Wegrzyn, Jill L.; Neale, David B.; Kirst, Matias; Peter, Gary F.; Gezan, Salvador A.; Nelson, C.Dana; Davis, John M. 2014. Mapping fusiform rust resistance genes within a complex mating design of loblolly pine. Forests. Forests 2014, 5, 347-362 16 p.
KeywordsPinus taeda, major gene resistance, gene-for-gene interaction, single nucleotide polymorphism (SNP), BayesC, Bayesian Association with Missing Data (BAMD), logarithm of the odds (LOD)
- Differential gene expression in loblolly pine (Pinus taeda L.) challenged with the fusiform rust fungus, Cronartium quercuum f.sp. fusiforme
- A genomic map enriched for markers linked to Avr1 in Cronartium quercuum f.sp. fusiforme
- Identification of nine pathotype-specific genes conferring resistance to fusiform rust in loblolly pine (Pinus taeda L.)
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