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    Author(s): K. J. Carim; T. Wilcox; M. K. YoungK. S. McKelveyM. K. Schwartz
    Date: 2015
    Source: Boise, ID: U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station, Boise Aquatic Sciences Lab. 10 p. Online: http://www.fs.fed.us/research/genomics-center/docs/edna/edna-protocol.pdf
    Publication Series: Miscellaneous
    Station: Rocky Mountain Research Station
    PDF: View PDF  (1010.0 KB)

    Description

    Throughout the 2014 field season, we had over two dozen biologist throughout the western US collect over 300 samples for eDNA analysis with paired controls. Control samples were collected by filtering 0.5 L of distilled water. No samples had any evidence of field contamination. This method of sampling verifies the cleanliness of the field equipment, as well as the handling methods of the field collector. However, it cannot measure any contamination that may occur in the process of collecting a sample from a stream or lake. For example, standing upstream of the sampling area in waders that have brook trout DNA on them while a sample is being collected may result in a false positive detection of brook trout in that sample. Similarly sampling for lake trout from a boat that was previously in a waterbody containing the fish may also contaminate a sample, leading to a false positive detection of lake trout in a field sample. As a result, we believe that a better approach for detecting false positives is to consider the local expert opinion of the area and verify results with repeated sampling where necessary.

    Related web site: Broad-scale genetic monitoring of aquatic species

    For most current version of publication: http://www.fs.fed.us/research/genomics-center/docs/edna/edna-protocol.pdf

    Publication Notes

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    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Carim, K. J.; Wilcox, T.; Young, M. K.; McKelvey, K. S.; Schwartz, M. K. 2015. Protocol for collecting eDNA samples from streams [Version 2.3]. Boise, ID: U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station, Boise Aquatic Sciences Lab. 10 p. Online: http://www.fs.fed.us/research/genomics-center/docs/edna/edna-protocol.pdf

    Keywords

    eDNA, field collection, sampling

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