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A consensus genetic map for Pinus taeda and Pinus elliottii and extent of linkage disequilibrium in two genotype-phenotype discovery populations of Pinua taeda

Author(s):

Jared W. Westbrook
Vikram E. Chhatre
Le-Shin Wu
Srikar Chamala
Leandro Gomide Neves
Patricio Munoz
Pedro J. Martinez-Garcia
David B. Neale
Matias Kirst
Keithanne Mockaitis
Gary F. Peter
John M. Davis

Year:

2015

Publication type:

Scientific Journal (JRNL)

Primary Station(s):

Southern Research Station

Source:

G3 Genes|Genomes|Genetics

Description

A consensus genetic map for Pinus taeda (loblolly pine) and Pinus elliottii (slash pine) was constructed by merging three previously published P. taeda maps with a map from a pseudo-backcross between P. elliottii and P. taeda. The consensus map positioned 3856 markers via genotyping of 1251 individuals from four pedigrees. It is the densest linkage map for a conifer to date. Average marker spacing was 0.6 cM and total map length was 2305 cM. Functional predictions of mapped genes were improved by aligning expressed sequence tags used for marker discovery to full-length P. taeda transcripts. Alignments to the P. taeda genome mapped 3305 scaffold sequences onto twelve linkage groups. The consensus genetic map was used to compare the genome-wide linkage disequilibrium in a population of distantly related P. taeda individuals (ADEPT2) used for association genetic studies and a multiple-family pedigree used for genomic selection (CCLONES). The prevalence and extent of LD was greater in CCLONES as compared to ADEPT2; however, extended LD with LGs or between LGs was rare in both populations. The average squared correlations, r2, between SNP alleles less than 1 cM apart was less than 0.05 in both populations and r2 did not decay substantially with genetic distance. The consensus map and analysis of linkage disequilibrium establish a foundation for comparative association mapping and genomic selection in P. taeda and P. elliottii.

Citation

Westbrook, Jared W.; Chhatre, Vikram E.; Wu, Le-Shin; Chamala, Srikar; Neves, Leandro Gomide; Munoz,Patricio; Martinez-Garcia, Pedro J.; Neale,David B.; Kirst, Matias; Mockaitis, Keithanne; Nelson, C. Dana; Peter, Gary F.; Davis, John M.; Echt, Craig S. 2015 A consensus genetic map for Pinus taeda and Pinus elliottii and extent of linkage disequilibrium in two genotype-phenotype discovery populations of Pinua taeda. G3.Genes/Genomes/Genetics Vol 5.1685-1694 10 p.

Cited

Publication Notes

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https://www.fs.usda.gov/treesearch/pubs/48896