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    Author(s): Zhonglian Huang; Peng Zhao; Jose Medina; Richard Meilan; Keith Woeste
    Date: 2013
    Source: PLoS ONE. 8(11): e75857. 14 p.
    Publication Series: Scientific Journal (JRNL)
    Station: Northern Research Station
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    Description

    An EST sequence, designated JnRAP2-like, was isolated from tissue at the heartwood/sapwood transition zone (TZ) in black walnut (Juglans nigra L). The deduced amino acid sequence of JnRAP2-like protein consists of a single AP2- containing domain with significant similarity to conserved AP2/ERF DNA-binding domains in other species. Based on multiple sequence alignment, JnRAP2-like appears to be an ortholog of RAP2.6L (At5g13330), which encodes an ethylene response element binding protein in Arabidopsis thaliana. Real-time PCR revealed that the JnRAP2-like was expressed most abundantly in TZ of trees harvested in fall when compared with other xylem tissues harvested in the fall or summer. Independent transgenic lines over-expressing JnRAP2-like in Arabidopsis developed dramatic ethylene-related phenotypes when treated with 50 μM methyl jasmonate (MeJA). Taken together, these results indicated that JnRAP2-like may participate in the integration of ethylene and jasmonate signals in the xylem and other tissues. Given the role of ethylene in heartwood formation, it is possible JnRAP2-like expression in the transition zone is part of the signal transduction pathway leading to heartwood formation in black walnut.

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    Citation

    Huang, Zhonglian; Zhao, Peng; Medina, Jose; Meilan, Richard; Woeste, Keith. 2013. Roles of JnRAP2.6-like from the transition zone of black walnut in hormone signaling. PLoS ONE. 8(11): e75857. 14 p.

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