Understanding environmental DNA detection probabilities: A case study using a stream-dwelling char Salvelinus fontinalisAuthor(s): Taylor M. Wilcox; Kevin S. McKelvey; Michael K. Young; Adam J. Sepulveda; Bradley B. Shepard; Stephen F. Jane; Andrew R. Whiteley; Winsor H. Lowe; Michael K. Schwartz
Source: Biological Conservation. 194: 209-216.
Publication Series: Scientific Journal (JRNL)
Station: Rocky Mountain Research Station
Download Publication (447.0 KB)
Related Research Highlights
The eDNAtlas project: A national map of aquatic biodiversity
Environmental DNA sampling (eDNA) has emerged as a powerful tool for detecting aquatic animals. Previous research suggests that eDNA methods are substantially more sensitive than traditional sampling. However, the factors influencing eDNA detection and the resulting sampling costs are still not well understood. Here we use multiple experiments to derive independent estimates of eDNA production rates and downstream persistence from brook trout (Salvelinus fontinalis) in streams. We use these estimates to parameterize models comparing the false negative detection rates of eDNA sampling and traditional backpack electrofishing. We find that using the protocols in this study eDNA had reasonable detection probabilities at extremely low animal densities (e.g., probability of detection 0.18 at densities of one fish per stream kilometer) and very high detection probabilities at population-level densities (e.g., probability of detection N0.99 at densities of ≥3 fish per 100 m). This is substantially more sensitive than traditional electrofishing for determining the presence of brook trout and may translate into important cost savings when animals are rare. Our findings are consistent with a growing body of literature showing that eDNA sampling is a powerful tool for the detection of aquatic species, particularly those that are rare and difficult to sample using traditional methods.
- You may send email to email@example.com to request a hard copy of this publication.
- (Please specify exactly which publication you are requesting and your mailing address.)
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationWilcox, Taylor M.; McKelvey, Kevin S.; Young, Michael K.; Sepulveda, Adam J.; Shepard, Bradley B.; Jane, Stephen F.; Whiteley, Andrew R.; Lowe, Winsor H.; Schwartz, Michael K. 2016. Understanding environmental DNA detection probabilities: A case study using a stream-dwelling char Salvelinus fontinalis. Biological Conservation. 194: 209-216.
KeywordseDNA, stream, detection, sampling, genetics, fish
- Environmental DNA particle size distribution from Brook Trout (Salvelinus fontinalis)
- Have brook trout (Salvelinus fontinalis) displaced bull trout (Salvelinus confluentus) along longitudinal gradients in central Idaho streams?
- Rainbow trout versus brook trout biomass and production under varied climate regimes in small southern Appalachian streams
XML: View XML