Sampling large geographic areas for rare species using environmental DNA: A study of bull trout Salvelinus confluentus occupancy in western MontanaAuthor(s): Kevin McKelvey; Michael Young; W. L. Knotek; K. J. Carim; T. M. Wilcox; T. M. Padgett-Stewart; Michael Schwartz
Source: Journal of Fish Biology. 88: 1215-1222.
Publication Series: Scientific Journal (JRNL)
Station: Rocky Mountain Research Station
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This study tested the efficacy of environmental DNA (eDNA) sampling to delineate the distribution of bull trout Salvelinus confluentus in headwater streams in western Montana, U.S.A. Surveys proved fast, reliable and sensitive: 124 samples were collected across five basins by a single crew in c. 8days. Results were largely consistent with past electrofishing, but, in a basin where S. confluentus were known to be scarce, eDNA samples indicated that S. confluentus were more broadly distributed than previously thought.
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CitationMcKelvey, K. S.; Young, M. K.; Knotek, W. L.; Carim, K. J.; Wilcox, T. M.; Padgett-Stewart, T. M.; Schwartz, M. K. 2016. Sampling large geographic areas for rare species using environmental DNA: A study of bull trout Salvelinus confluentus occupancy in western Montana. Journal of Fish Biology. 88: 1215-1222.
Keywordsdistribution, eDNA, genetics, occupancy, quantitative PCR
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