Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub

    Description

    We have used thermospray LC-MS to confirm three highly polar metabolites (A, B. and G) of the herbicide hexazinone [3-cyclohexyl-6-(dimethylamino)-l-methyl-1,3,5-triazine-2,4(1H,3H)-dione]. and chemical ionization GC-MS to confirm two other metabolites (D and E) in extracts of soil and vegetation from a forest in the Central Alabama Piedmont. Selected-ion monitoring (SIM) of the protonated molecular ions of metabolite A [3-(4-hydroxycyclohexyl)-6-(dimethylamino)-1-methyl-1,3.5-triazine-2.4( 1H.3H)-dione] at mass-to-charge ratio (m/z) 269 and metabolite B [3-cyclohexy1-6-(methylamino)-1-methyl-1,3,5-triazine-2.4( 1H,3H)-dione] at m/z 239 gave matrix detection limits (MDLs) of 25 ppb (ngig) and 50 ppb. respectively, in 10 g vegetation samples. MS quanquantitation fortitation for A and B generally confirmed high-performance liquid chromatography data. SIM of the protonated molecular ion of metabolite G [3-cyclohexyl-6-(methylamino)-1,3,5-triazine-2,4(1H,3H)-dione] at m/z 225 afforded an MDL of 5 ppb in 50 g topsoil samples. Metabolite G was not confirmed above the MDL in any of the soils tested. Chemical ionization GC-MS using methane reagent gas gave strong ion signals for metabolite D [3-cyclohexyl-1-methyl-1,3,5-triazine-2,4,6(1H,3H,SH)-trione] at m/z 226 and 144 and metabolite E (3-(4-hydroxycyclohexyl)-l-methyl-1,3,5-triazine-2,4,6(1H,3H,SH)-trione] at m/z 242, 224. and 144. SIM at these masses afforded MDLs of 50 ppb for D and 400 ppb for E in 10 g vegetation samples,

    Publication Notes

    • You may send email to pubrequest@fs.fed.us to request a hard copy of this publication.
    • (Please specify exactly which publication you are requesting and your mailing address.)
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Fischer, Joseph B.; Michael, Jerry L. 1995. Thermospray ionization liquid chromatography-mass spectrometry and chemical ionization gas chromatography- mass spectrometry of hexazinone metabolites in soil and vegetation extracts. Journal of Chromatography A. 704: 131-139

    Related Search


    XML: View XML
Show More
Show Fewer
Jump to Top of Page
https://www.fs.usda.gov/treesearch/pubs/5019