Skip to Main Content
DNA barcoding of gypsy moths from China (Lepidoptera: Erebidae) reveals new haplotypes and divergence patterns within gypsy moth subspeciesAuthor(s): Fang Chen; Youqing Luo; Melody A. Keena; Ying Wu; Peng Wu; Juan Shi
Source: Journal of Economic Entomology. 109(1): 366-374.
Publication Series: Scientific Journal (JRNL)
Station: Northern Research Station
Download Publication (577.0 KB)
Related Research Highlights
Elevated Nitrogen Deposition Changes Herb Layer Diversity
DescriptionThe gypsy moth from Asia (two subspecies) is considered a greater threat to North America than European gypsy moth, because of a broader host range and females being capable of flight. Variation within and among gypsy moths from China (nine locations), one of the native countries of Asian gypsy moth, were compared using DNA barcode sequences (658 bp of mtDNA cytochrome c oxidase subunit 1 [COI] sequence), together with two restriction site mtDNA markers (NlaIII and BamHI in COI), which is the standard system used to distinguish European gypsy moths from Asian gypsy moths. Relatedness of these populations to gypsy moths from seven other world areas was also examined. The restriction site markers showed that two Chinese populations had both Asian and European haplotypes. DNA barcode sequence divergence between the Asian populations and the European populations was three times greater than the variation within each group. Using Bayesian and parsimonious network analyses, nine previously unknown barcode haplotypes were documented from China and a single haplotype was found to be shared by 55% of the Chinese and some Far Eastern Russian and Japanese individuals. Some gypsy moths from two Chinese populations showed genetic affinity with mtDNA haplotypes from Siberia, Russia, suggesting there could be a cryptic new subspecies in Lymantria dispar (L.) or human-aided movement of moths between these two locations at an earlier point in time. The previously unknown haplotype patterns may complicate efforts to identify Asian gypsy moth introductions and require changes in monitoring and exclusion programs.
- Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat.
- During the capture process some typographical errors may occur.
- Please contact Sharon Hobrla, email@example.com if you notice any errors which make this publication unusable.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationChen, Fang; Luo, Youqing; Keena, Melody A.; Wu, Ying; Wu, Peng; Shi, Juan. 2015. DNA Barcoding of Gypsy Moths From China (Lepidoptera: Erebidae) Reveals New Haplotypes and Divergence Patterns Within Gypsy Moth Subspecies. Journal of Economic Entomology. 109(1): 366-374.
Keywordsgypsy moth, COI, NB system, DNA barcoding, haplotype
- Assessing the potential of genotyping-by-sequencing-derived single nucleotide polymorphisms to identify the geographic origins of intercepted gypsy moth (Lymantria dispar) specimens: A proof-of-concept study
- Comparison of Survival and Development of Gypsy Moth Lymantria dispar L. (Lepidoptera: Erebidae) Populations from Different Geographic Areas on North American Conifers
- Identification and characterization of a RAPD-PCR marker for distinguishing Asian and North American gypsy moths
XML: View XML